Purpose Spaceflight impairs physical capacity. Here we assessed the protective effect of artificial gravity (AG) on aerobic exercise capacity and muscle function during bed rest, a spaceflight analogue. Methods 24 participants (33 ± 9 years, 175 ± 9 cm, 74 ± 10 kg, 8 women) were randomly allocated to one of three groups: continuous AG (cAG), intermittent AG (iAG) or control (CTRL). All participants were subjected to 60 days of six-degree head-down tilt bed rest, and subjects of the intervention groups completed 30 min of centrifugation per day: cAG continuously and iAG for 6 × 5 min, with an acceleration of 1g at the center of mass. Physical capacity was assessed before and after bed rest via maximal voluntary contractions, cycling spiroergometry, and countermovement jumps. Results AG had no significant effect on aerobic exercise capacity, flexor muscle function and isometric knee extension strength or rate of force development (RFD). However, AG mitigated the effects of bed rest on jumping power (group * time interaction of the rmANOVA p < 0.001; iAG − 25%, cAG − 26%, CTRL − 33%), plantar flexion strength (group * time p = 0.003; iAG − 35%, cAG − 31%, CTRL − 48%) and plantar flexion RFD (group * time p = 0.020; iAG − 28%, cAG − 12%, CTRL − 40%). Women showed more pronounced losses than men in jumping power (p < 0.001) and knee extension strength (p = 0.010). Conclusion The AG protocols were not suitable to maintain aerobic exercise capacity, probably due to the very low cardiorespiratory demand of this intervention. However, they mitigated some losses in muscle function, potentially due to the low-intensity muscle contractions during centrifugation used to avoid presyncope.

Purpose: Long stays in space require countermeasures for the degrading effects of weightlessness on the human body, and artificial gravity (AG) has been proposed as an integrated countermeasure. The aim of this study was to assess the cardiorespiratory and neuromuscular demand of AG elicited via daily centrifugation during 60 days of bed rest. Methods: Twenty four participants (33 ± 9 y, 175 ± 9 cm, 74 ± 10 kg, 8 female) were subjected to 60 days of strict six-degree head-down tilt (HDT) bed rest and were randomly allocated to one of three experimental groups: 30 min of daily centrifugation with an acceleration of 1 g at the center of mass and 2 g at the feet applied continuously (cAG) or intermittently in 6 epochs of 5 min each, separated by 3 min breaks (iAG), or non-centrifuged control (CTRL). Cardiorespiratory demand during centrifugation was assessed at the beginning (HDT3) and end (HDT60) of the bed rest phase via spirometry and heart rate monitoring, leg muscle activation was monitored via electromyography. Results: On average, analyses of variance revealed that heart rate during centrifugation increased by 40% (iAG) and 60% (cAG) compared to resting values (p < 0.001), while oxygen uptake did not change significantly (p = 0.96). There was a preference for calf over knee extensor muscle activation (active time soleus 57 ± 27%, gastrocnemius medialis 45 ± 27% and vastus lateralis 27 ± 27%, p < 0.001), with large inter-individual differences in leg muscle active time. AG could not prevent the increase in resting heart rate after bed rest. For most of the recorded parameters, there were little differences between cAG and iAG, with the increase in heart rate during centrifugation being a notable exception (greater increase for cAG, p = 0.01). Conclusion: Daily 30 min bouts of artificial gravity elicited by centrifugation put a substantial demand on the heart as a pump without increasing oxygen consumption. If centrifugation is to be used as a countermeasure for the deteriorating effects of microgravity on physical performance, we recommend combining it with strenuous exercise.

Several studies indicate that acute exercise induces DNA damage, whereas regular exercise increases DNA repair kinetics. Although the molecular mechanisms are not completely understood, the induction of endogenous reactive oxygen species (ROS) during acute exhaustive exercise due to metabolic processes might be responsible for the observed DNA damage, while an adaptive increase in antioxidant capacity due to regular physical activity seems to play an important protective role. However, the protective effect of physical activity on exogenously induced DNA damage in human immune cells has been poorly investigated. We asked the question whether individuals with a high aerobic capacity would have an enhanced response to radiation-induced DNA damage. Immune cells are highly sensitive to radiation and exercise affects lymphocyte dynamics and immune function. Therefore, we measured endogenous and radiation-induced DNA strand breaks and poly (ADP-ribose) polymerase-1 (PARP1) activity in peripheral blood mononuclear cells (PBMCs) from endurance-trained (maximum rate of oxygen consumption measured during incremental exercise V’O2max > 55 mL/min/kg) and untrained (V’O2max < 45 mL/min/kg) young healthy male volunteers before and after exhaustive exercise. Our results indicate that: (i) acute exercise induces DNA strand breaks in lymphocytes only in untrained individuals, (ii) following acute exercise, trained individuals repaired radiation-induced DNA strand breaks faster than untrained individuals, and (iii) trained subjects retained a higher level of radiation-induced PARP1 activity after acute exercise. The results of the present study indicate that increased aerobic fitness can protect immune cells against radiation-induced DNA strand breaks.

This study evaluated the reliability and sensitivity of a set of different common strength and power tests in a healthy adult population in a span of 9 weeks. Seventeen subjects (24.2 ± 2.2 years, 1.75 ± 0.10 m, 68.6 ± 14.2 kg, seven women) participated in the study. We tested countermovement jumps, reactive hops, and the maximal voluntary contraction (MVC) of handgrip and isometric knee extension. The tests were conducted in three separate sessions across a nine-week period, with one week between the first two sessions and eight weeks between the second and the third. Reliability and sensitivity statistics for each test were calculated for both the average of three trials and the best result during each session. The MVC of isometric knee extension and handgrip, as well as the countermovement jump test, demonstrated very high reliability and sensitivity over the nine-week period. The peak force of the reactive hops demonstrated high reliability but high sensitivity only for the average but not for the best result. The average contact time of reactive hops was neither a sensitive nor reliable measurement. In conclusion, isometric maximal knee extension and handgrip tests, as well as countermovement jumps and peak force of reactive hops, can be used as reliable and sensitive measurements of isometric and reactive strength and power over time periods of up to eight weeks. We recommend the use of the average results of three trials instead of the best performance value for longitudinal studies, as this procedure produces more consistent results and a lower measurement error.

Purpose High-intensity interval training (HIIT) has proven to be effective in improving endurance capacity and muscle endurance. However, its potential to improve other aspects of physical performance such as strength and power has not been well explored, and most research studies used only running and cycling as exercise modalities. Here, we compared the effects of jumping versus running as exercise modalities during a 6-week HIIT. Methods 46 participants (24±3 years, 171±9 cm, 68±13 kg, 22 women) were randomly allocated to one of three groups: countermovement jump training, running training, or control. The two training groups underwent a 6-week HIIT with 3 training sessions per week. Both training protocols had identical training frequency, number of series and work/rest durations (on average 7 series of 25s, with a rest of 25s between series). Before and after the training period, aerobic capacity and neuromuscular performance were assessed. Results Analyses of variance revealed a significant group*time interaction effect for maximal aerobic capacity (p = 0.004), and post hoc analyses showed a significant increase in the running group (p < .001, +7.6%). Analyses of the maximal voluntary contraction revealed only a significant increase in the jumping group (plantar flexion +12.8%, knee extension +8.2%). No interaction effects were found for maximal power or jump height. Conclusion Despite identical programming, the choice of exercise mode profoundly affected the training adaptations: the running group significantly increased aerobic capacity, and the jump group significantly increased leg strength. These results underline the importance of exercise modality in physical performance adaptations.