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Fragile X syndrome (FXS) is the first cause of inherited intellectual disability, due to the silencing of the X-linked Fragile X Mental Retardation 1 gene encoding the RNA-binding protein FMRP. While extensive studies have focused on the cellular and molecular basis of FXS, neither human Fragile X patients nor the mouse model of FXS-the Fmr1-null mouse-have been profiled systematically at the metabolic and neurochemical level to provide a complementary perspective on the current, yet scattered, knowledge of FXS. Using proton high-resolution magic angle spinning nuclear magnetic resonance ( 1 H HR-MAS NMR)-based metabolic profiling, we have identified a metabolic signature and biomarkers associated with FXS in various brain regions of Fmr1-deficient mice. Our study highlights for the first time that Fmr1 gene inactivation has profound, albeit coordinated consequences in brain metabolism leading to alterations in: (1) neurotransmitter levels, (2) osmoregulation, (3) energy metabolism, and (4) oxidative stress response. To functionally connect Fmr1-deficiency to its metabolic biomarkers, we derived a functional interaction network based on the existing knowledge (literature and databases) and show that the FXS metabolic response is initiated by distinct mRNA targets and proteins interacting with FMRP, and then relayed by numerous regulatory proteins. This novel ''integrated metabolome and interactome mapping'' (iMIM) approach advantageously unifies novel metabolic findings with previously unrelated knowledge and highlights the contribution of novel cellular pathways to the pathophysiology of FXS. These metabolomic and integrative systems biology strategies will contribute to the development of potential drug targets and novel therapeutic interventions, which will eventually benefit FXS patients.[Supplemental material is available for this article.]Fragile X syndrome (FXS) is the most frequent cause of inherited intellectual disability (ID) and the most commonly identified genetic cause of autism (Chelly et al. 2006;Gecz et al. 2009). FXS affects 1 in 4000 males and 1 in 7000 females world-wide (Hagerman 2008) and is caused by the silencing of the X-linked Fragile X Mental Retardation 1 (FMR1) gene positioned in Xq27.3. In FXS patients, a dynamic mutation increasing abnormally the number of CGG repeats in the first exon of the FMR1 gene leads to their hypermethylation and the subsequent absence of its gene product, FMRP (Penagarikano et al. 2007). Although a monogenic disorder, FXS is a disease of complex etiology accompanied by behavioral (hyperactivity, autism), neurological (susceptibility to epileptic seizures), as well as physical abnormalities (macroorchidism, elongated face, hyperextensible finger joints) (Penagarikano et al. 2007). A mouse model of FXS knock-out (KO) for the murine homolog of FMR1, has been generated, exhibiting learning and behavioral abnormalities that recapitulate the human phenotype (The Dutch-Belgian Fragile X Consortium 1994). The absence of FMRP induces abnormal extra dendritic spines...

Fragile X (FRAX) syndrome is a common inherited form of mental retardation resulting from the lack of fragile X mental retardation protein (FMRP) expression. The consequences of FMRP absence in the mechanism underlying mental retardation are unknown. Here, we tested the hypothesis that glutamate receptor (GluR) expression might be altered in FRAX syndrome. Initial in situ hybridization and Western blotting experiments did not reveal differences in mRNA levels and protein expression of AMPA and NMDA subunits and metabotropic glutamate subtype 5 (mGlu5) receptors between control and Fmr1 knock-out (KO) mice during postnatal development. However, a detergent treatment (1% Triton X-100) revealed a selective reduction of mGlu5 receptor expression in the detergent-insoluble fraction of synaptic plasma membranes (SPMs) from KO mice, with no difference in the expression of NR2A, GluR1, GluR2/3, GluR4, and Homer proteins. mGlu5 receptor expression was also lower in Homer immunoprecipitates from Fmr1 KO SPMs. Homer, but not NR2A, mGlu5, and GluR1, was found to be less tyrosine phosphorylated in Fmr1 KO than control mice. Our data indicate that, in FRAX syndrome, a reduced number of mGlu5 receptors are tightly linked to the constituents of postsynaptic density and, in particular, to the constitutive forms of Homer proteins, with possible consequent alterations in synaptic plasticity.

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