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The freezing of fish at (-18°C) in the freezer compartment of a domestic refrigerator for 2, 4, 6 and 8 weeks influenced the biochemical composition and mineral contents of the muscles. The chemical analysis of the muscles of the studied fish recorded high values (% dry weight) of protein, fat and ash contents and moisture for the fresh samples and there was a significant (P<0.05) decrease at the end of the eight weeks of freezing fish. The relationship between the four components of fresh and frozen Tilapia muscles was detected. There was only a significant (P<0.05) relationship between protein and fat contents (0.999) and between moisture and ash contents (0.998) after the 8 weeks of freezing, as for the mineral content of the frozen fish. The maximum values recorded throughout the freezing period were after 2 weeks of freezing (Cu, Ca, K, and Zn); after 4 weeks (P ,Na and K) and after 8 weeks (Mg, Fe and Na). In conclusion, the present results determinate the quality changes during storage period (8 weeks) and how long fish muscle can be stored without any deterioration in a domestic refrigerator.

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F enthion is an organophosphate pesticide, widely used all over the world as a broad-spectrum insecticide for the protection of numerous crops especially rice. Due to these agricultural activities Fenthion has repeatedly been detected in surface waters and soil. Thus. Fenthion is among the most toxic pesticides for aquatic life especially fish. This study had investigated die toxic effect of Fenthion on some immunological and histopathological parameters. Fish were exposed to concentrations of 0.00 (control). 0.S3 ppm and 2.1 ppm for 96 hours. The histopathological alterations for lymphoid and non-lymphoid organs, as compared to the control group. included hemosiderosis and atrophy in spleen and pronephrous. In gills, hemorrhage, progressive degeneration and lymphocj le infiltration were reported: in liver and pancreas atrophy has been shown in addition to excessive zvmosen granules secretion in pancreas. The kidney trunk showed glomerular and tubular atrophy and lymphocyte inilltration. For the alterations of plasma immunoglobulin level. Fenthion at all concentrations suppressed plasma IgM level (P< 0.05). So it can be concluded that Fenthion causes adverse effects on immunological and histopathological parameters of exposed fish.