Streptococcus pasteurianus, also known as S. gallolyticus subsp. pasteurianus (SGSP), is a Gram-positive coccoid bacterium that belongs to S. bovis group and was previously identified as S. bovis biotype II/2. 1 This group of bacteria is responsible for human infections, resulting in endocarditis, bacteremia, hepatobiliary disease, colonic lesions, and neonatal meningitis. [1][2][3][4] SGSP is also considered an underreported zoonotic pathogen, being isolated from piglet's brain with meningitis 5 and from goslings and turkeys with neurological signs and sepsis. [6][7][8] There is no previous report of SGSP infection in a non-human primate. Herein, we describe a case of aortic valvular endocarditis with sepsis, caused by SGSP in a puerperal captive emperor tamarin (Saguinus imperator).