Microbial fuel cells (MFCs) are electrochemical devices focused on bioenergy generation and organic matter removal carried out by microorganisms under anoxic environments. In these types of systems, the anodic oxidation reaction is catalyzed by anaerobic microorganisms, while the cathodic reduction reaction can be carried out biotically or abiotically. Membranes as separators in MFCs are the primary requirements for optimal electrochemical and microbiological performance. MFC configuration and operation are similar to those of proton-exchange membrane fuel cells (PEMFCs)—both having at least one anode and one cathode split by a membrane or separator. The Nafion® 117 (NF-117) membrane, made from perfluorosulfonic acid, is a membrane used as a separator in PEMFCs. By analogy of the operation between electrochemical systems and MFCs, NF-117 membranes have been widely used as separators in MFCs. The main disadvantage of this type of membrane is its high cost; membranes in MFCs can represent up to 60% of the MFC’s total cost. This is one of the challenges in scaling up MFCs: finding alternative membranes or separators with low cost and good electrochemical characteristics. The aim of this work is to critically review state-of-the-art membranes and separators used in MFCs. The scope of this review includes: (i) membrane functions in MFCs, (ii) most-used membranes, (iii) membrane cost and efficiency, and (iv) membrane-less MFCs. Currently, there are at least 20 different membranes or separators proposed and evaluated for MFCs, from basic salt bridges to advanced synthetic polymer-based membranes, including ceramic and unconventional separator materials. Studies focusing on either low cost or the use of natural polymers for proton-exchange membranes (PEM) are still scarce. Alternatively, in some works, MFCs have been operated without membranes; however, significant decrements in Coulombic efficiency were found. As the type of membrane affects the performance and total cost of MFCs, it is recommended that research efforts are increased in order to develop new, more economic membranes that exhibit favorable properties and allow for satisfactory cell performance at the same time. The current state of the art of membranes for MFCs addressed in this review will undoubtedly serve as a key insight for future research related to this topic.

Acid mine drainage (AMD) is a source of soil and water resources pollution. Calcite is a mineral constituted of calcium carbonate (CaCO3). The AMD interaction with calcite drives their natural neutralization. Calcite is the main component of the chicken eggshell (ES). This work aimed to evaluate the use of ES waste as a material to treat raw AMD. Five treatments (T1, T2, T3, T4, and T5) were carried out with concentrations of 1, 2, 3, 4, and 5 ES g/L AMD, respectively. Each treatment was performed for 3 h at room temperature without agitation. The response variables analyzed were pH, redox potential (Eh), electrical conductivity (σ), chlorides (Cl–), alkalinity, sulfates (SO42–), nitrates (NO3–, and potentially toxic heavy metals and metalloids (PTHMM). Also, the removal efficiencies of SO42–, NO3–, and PTHMM were analyzed. Additionally, the chemical and mineralogical composition of ES and precipitates were determined. The initial pH for AMD was 2.50 and it reached a final value of 5.50, 5.60, 5.80, 5.93, and 6.12 in T1, T2, T3, T4 and, T5, respectively. Moreover, the different treatments granted alkalinity to the treated effluents, reaching a maximum value of 124 CaCO3 mg/L in T5. Finally, Al and Fe were completely removed from AMD, whereas Cu reached > 95 % removal, especially in T3, T4, and T5. Ba, Cr, and Pb showed an average removal of ~65 %. The ES concentration that showed the best results of neutralization and PTHMM removal efficiency was 5 ES g/L. The results showed that ES is a biocompatible waste material with an added value because it can be used as a sustainable material to treat raw AMD.

The management and disposal of the sewage sludge (SS) generated by a wastewater treatment plant (WWTP) as part of the municipal wastewater (MWW) treatment process is one of the main socio-environmental issues faced by this type of system. Taxco de Alarcón, Guerrero, in southern Mexico has had a WWTP operating since 2016, and the SS disposal is a task that must be addressed by the WWTP. Thus, the aim of this work was to evaluate the growth capacity of Panicum maximum, also known as mombaza grass (MG), by using SS generated within the "Taxco de Alarcón wastewater treatment plant" as substrate. To do so, 4 g of MG seeds were scattered over 5 kg (dry basis) of SS. As a control, a commercial compost soil was used, hereafter called pattern soil (PS). The experiment was carried out in triplicates for three months and drinking water (water used for human consumption) was used for crop irrigation. Each month a MG harvest was carried out. The response variables analyzed for MG were germination time (one month after plant emergence), height (HMG), growth rate GrMG, and yield (YMG), whereas in the SS and PS the content of organic matter was analyzed. Furthermore, the chemical composition was analyzed using scanning electron microscopy and X-ray energy dispersion spectroscopy (SEM-EDS) on the MG, SS, and PS. The results showed that MG germinated faster on PS (5 days) than germination on SS (7 days). However, the MG grown on SS reached a considerably higher height (45 cm) compared to the height reached on PS (17 cm). Furthermore, the maximum GrMG over SS was also higher than the maximum GrMG observed on the PS, 3.64 and 1.40 cm∙day-1, respectively. In terms of YMG, it was observed that on SS it reached an average monthly YMG of 416 g∙m-2, whereas in PS it reached a YMG of 72 g∙m-2. The chemical analysis detected P, K, Ca, Mg, and S, considered macronutrients in both substrates. Besides, some micronutrients identified in SS were Cu, Fe, Mn, and Zn, whereas in PS it was also possible to detect micronutrients except Mn and Zn. All the macronutrients detected in the substrates were observed in the harvested MG. However, in the MG harvested in PS, Mn and Zn were not detected. Hence, a feasible disposal strategy for the SS generated by the Taxco de Alarcón WWTP is as a substrate for grass forage MG by its high organic matter content, the significant presence of macro and micronutrients, and the performance shown by MG cultivated in SS. Furthermore, the SS characteristics provide added value and can be considered as organic amendments of agricultural soils.

Population growth increases the challenge of meeting basic human needs, such as water, a limited resource. Consumption habits and water pollution have compromised natural resources to unsustainable levels. Sustainable effluent treatment practices, such as decentralized systems focused on energy, nutrients, and water recovery, have attracted the attention of the scientific community. Human urine (HU) is a physiological liquid waste whose main component is water (~95%). HU has a significant amount of nutrients, such as N, P, K, and organic matter, which are usually lacking in fecal coliforms. Therefore, the possibility exists of recovering nutrients and energy from HU using sustainable and non-sustainable technologies. Treating HU in bioelectrochemical systems (BES) is a novel alternative to obtaining byproducts from this effluent more sustainably than in electrochemical systems. Microbial fuel cells (MFCs) are an interesting example, contributing to HU revalorization from unwanted waste into a valuable resource of nutrients, energy, and water. Even when urine-operated MFCs have not generated attractive potential outputs or produced considerable amounts of bioelectricity, this review emphasizes HU advantages as nutrients or water sources. The aim of this review was to analyze the current development of BES for HU treatment based on the water circular economy, discussing challenges and perspectives researchers might encounter.

Climate change, urbanization, and population growth, particularly in urban areas such as Acapulco, Mexico, put pressure on water availability, where although surrounded by water, the inhabitants lack enough good-quality water, especially in the rainy season. In addition, water scarcity, socioeconomic factors, and infrastructure problems limit the satisfaction of water demand in this context, e.g., operational issues in the water treatment plants and problems in the distribution network caused by hurricanes. The objectives of this research were: (i) to determine the rainwater quality in Acapulco, Mexico; (ii) to propose a domestic water efficiency retrofit (WER) design implementing a rainwater harvesting system (RWHS); and (iii) to determine the RWHS efficiency in terms of economic savings, considering rainwater’s social acceptance for domestic consumptive uses. The WER design was developed in an SFH in Acapulco, Mexico. The RWHS catchment surface area was 29 m2. The device comprises a first-rain separator (20 L) and a storage tank (1200 L). The rainwater harvesting potential (RWHP) was evaluated during the 2020 and 2021 rainy seasons, whereas the harvested rainwater quality (HRWQ) was analyzed in samples from 2021. Alkalinity, pH, electrical conductivity, total dissolved solids, chlorides, nitrates, sulfates, and heavy metals and potentially toxic metalloids were analyzed. Additionally, 168 surveys were applied to SFH owners to evaluate WER acceptance. Results showed that the RWHP was ca. 44 and 21 L/m2 in 2020 and 2021, respectively. All the rainwater quality parameters met the World Health Organization guidelines for consumptive uses except for drinking water. The perception study showed a 95% willingness to adopt the WER. Due to the RWHP and the HRWQ, the WER of SFHs is a promising solution to address Acapulco hydric stress under the nature-based solutions approach.