Objective: To verify the prevalence of intestinal parasitic infections among preschoolers and to identify the associated risk factors. Methods: The study is a cross-sectional study nested in a cohort of children who were born and resident in Diamantina, Minas Gerais, Brazil. At the time of the study, all children were aged 60 months 6 five months. They were recruited after written informed consent was obtained from parents or guardians. The study was carried out between July 2009 and July 2010. In total 214 children provided a stool sample for examination on intestinal parasitic infections. Information on potential risk factors for parasitosis was obtained from parents and guardians of the children by a questionnaire. Logistic regression was used for analysis. Results: Intestinal parasitic infections were found in 27.5% (n559) of children. The boys' infection prevalence (26.1%, n536) was slightly lower than the infection prevalence of the girls (30.3%, n523), but not statistically different (p50.51). Fourteen children, (23.7%) were infected with two or more parasite species and forty-five (76.3%) with single parasites. A low per capita income of family was strongly associated with an increased risk for an infection (OR52.89; P50.003). Preschoolers whose mothers did not work outside home had a significantly lower risk for infection (OR50.41; p50.01).