In planta the protein is predominantly present in its phosphorylated form, but it is rapidly dephosphorylated during isolation under native conditions. In an effort to examine the nature of the protein kinase(s) involved in the phosphorylation reaction, prl7 deletion mutants were expressed as fusion proteins in a bacterial expression vector system and tested for their ability to be phosphorylated by potato membrane preparations as well as by commercially available kinases. A fusion protein containing the nucleic acid-binding, basic, C-proximal domain (prl7Cl) was identified to be phosphorylated by a Ca 2+ -and phospholipiddependent, membrane-associated protein kinase. This protein kinase activity was inhibited by the addition of (19-36) protein kinase C (PKC) inhibitory peptide, known to be a highly specific inhibitor of mammalian PKC. Moreover, also the mammalian PKC from rat was able to phosphorylate prl7 in vitro. The results suggest that phosphorylation of prl7 takes place at membranous structures, possibly at the deltoid plasmodesmata connecting the sieve cell-companion cell complex of the phloem, by the activity of PKC-related, membrane-associated protein kinase activity.
In recent decades, the activities of major American IT companies have significantly increased the risks in the field of information security and personal data protection in the United States, revealing the national legislation gap regarding the development of modern technologies. On the one hand, privacy has been violated by the IT companies, whose servers store personal data of millions of citizens from different countries. On the other hand, US law enforcement agencies have been seeking to expand their functions to use this data for national security purposes. In this situation, there is a question of new legal regulatory approaches that meet the requirements of the modern technological environment. The new European model of personal data protection (General data protection regulation, GDPR) has a great influence on this process.
The article examines the prospects and risks associated with the development of quantum technologies in the perspective of the US-China technological rivalry. In the coming decades, quantum technologies will act as a driving force for technological development, and the priority in the development and use of this innovative technology will pave the way for global technological leadership in national security, digital economy, military and defense industries. A number of future risks are predicted, among them those related to the socially fair use of quantum technologies, geopolitical and national security aspects of their use, and the need to create regulatory mechanisms (including international ones) and standards for quantum technologies. The question is raised about the current ethical initiatives of the IT and business communities to prevent the risks associated with quantum technologies in the future.
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