The aim of this study was to apply the Alamar Blue assay to determine the susceptibility to antituberculosis pharmaceuticals, in Mycobacterium tuberculosis isolated strains from patients with tuberculosis (TB). In this study, a TB diagnostic was done by bacilluscope as an essential diagnostic tool for pulmonary TB using the Ziehl-Neelsen (ZN) stain. Furthermore, a culture was prepared in Lowenstein-Jensen (L-J) solid media, Middlebrook 7H9, and Middlebrook 7H10. For all the clinical samples investigated, five reference strains of M. tuberculosis were used to standardize the sensibility to streptomycin (SM), isoniazid (INH), rifampicin (RIF), and ethambutol (EMB) of the ATCC. Additionally, 97 Mycobacterium strains were analyzed from clinical isolations. The microplate Alamar Blue assay (MABA) was standardized and applied. The resistance levels of this disease were obtained in La Comarca Region (Lagunera). The results indicated that the MABA test is fast and easy to apply, however the most important aspect is the reliability of the method to determine the drug sensibility to pharmaceuticals. The MABA colorimetric test can be used in different regional as well as state TB diagnostic laboratories.

The activation and nuclear translocation of NF-B and the expression of the pro-inflammatory cytokine genes by macrophages infected with the attenuated Brucella abortus RB51 and virulent 2308 strains were evaluated. pIBα and NF-B were determined by immunoblot, and cytokines IFN- and IL12 mRNA were determined by reverse transcriptase polymerase chain reaction (qPCR) and translocation of NF-B protein to the nucleus was determined by electrophoretic mobility shift assay (EMSA). We demonstrate that the attenuated B. abortus RB51 strain stimulates cells resulting in NF-B activation and nuclear translocation, during experimental infection in macrophages J774A.1 which induced a pro-inflammatory response producing IL-6, 12 TNF-s INF-g and iNOS. The virulent strain B. abortus 2308 also stimulated the cells but induced a p50 homodimer of NF-B which is inactive. The p50 homodimer of NF-B binds to DNA, and thus blocked the activation of pro-inflammatory cytokines genes. Therefore, an evasion mechanism of the strain 2308 is to produce an inactive homodimer of NF-B which does not give rise to pro-inflammatory response to eliminate the bacteria.

Platelet-rich plasma (PRP) is a reliable source for obtaining cells to regenerate tissues, with ease of availability inorder to implement and standardize the ideal methodology in centrifugation strength and time for obtaining therapeutic-quality PRP, allowing its application to provide better and rapid recovery of muscular injuries, tendinitis, bone and ligament lesions. To evaluate PRP therapy, 150 patients with muscular lesions, tendinitis, shoulder, knee, ankle, hand and elbow injuries were treated. On application of PRP, we obtained 100% clinically significant symptomatic improvement in all 150 patients treated, who had musculoskeletal and ligament injuries, with a marked reduction of pain and inflammation. We concluded that the ideal concentration for obtaining PRP is at 1000 rpm with a time of 5 min; in addition, under these conditions the plasma lacks leukocytes and erythrocytes. The results were reproducible because the experiment was repeated at two institutions under the same conditions and similar results were obtained. The regeneration obtained in the affected patients is due to the fact that growth factors were released from the activated platelets; these initiate and modulate cicatrization in the tissues, which is a recent innovation to promote cicatrization, accelerating the power of tissue regeneration, with a platelet concentrate suspended in plasma.