Teaching Biochemistry courses is extremely difficult in preclinical medical education because of monotonous use of lectures, tutorials, practical and end semester load with end semester examination which is a burden for undergraduates. Purposes of this study are to identify the effectiveness of teaching methods and implementation of the best method of teaching Biochemistry course. Participants of this study were second year medical student (n=177) of Faculty of Medicine, University of Rajarata, Sri Lanka. Two non-compulsory evaluating questions were administered with each of the candidates when they sat for 2nd MBBS Objective Structured Practical Examination (OSPE). The students gave high positive rating for the lectures. The preferred order of the teaching method includes lectures as the first preference and other methods include Student Staff interaction, panel discussion and the least preference was seminar. To conclude it is found that still students prefer lectures because they get more knowledge easily with face to face contact.