Flower head orders and the use of GA3 (gibberellic acid) treatment could be two influencing factors determining the bioactive compound levels in artichoke, but little to no information is available about their effects. In this study, we have therefore evaluated the influence of these factors on the hydroxycinnamic acid and luteolin derivative levels in three categories of artichoke: Seed-propagated open-pollinated cultivars; vegetatively propagated cultivars; and seed-propagated hybrids. The hydroxycinnamic acids and luteolin derivatives were quantified by RP-HPLC-DAD. The average flower head weight was the lowest in tertiary heads and GA3-treated artichokes, followed by secondary and main heads. Moreover, the hydroxycinnamic acid and luteolin derivatives levels were significantly higher in tertiary heads than in secondary or main heads. In addition, the GA3 treatment significantly reduced the hydroxycinnamic acid content and, in contrast, improved luteolin derivatives levels. These effects depended on the flower head order and cultivar. Knowledge of the effects of flower head order and GA3 treatment is therefore key in order to achieve the greatest health-benefits from artichoke consumption.
Previous research works have reported that ‘Lorca’ artichoke cultivar presents a lower total phenolic content than other cultivars rich in phenolic compounds, which could show a lower susceptibility to enzymatic browning and increase its aptitude for fresh-cut processing. The aim of this study was to analyze the total phenolic content as well as browning evaluation by image analysis and polyphenol oxidase (PPO) enzyme activity in ‘Lorca’ cultivar in order to characterize the key factors which influence its phenolic levels for minimally processed artichokes. Thus, artichokes were harvested and classified on three head orders (main, secondary, and tertiary), as well as three development stages (initial, intermediate, and advanced). Variance components analysis was carried out for total phenolic content considering three factors: plant, flower head order, and internal development stage. For the first time, the internal development stage has been related to total phenolic content, and results showed that artichoke head order and internal development stage were responsible for a variability of 22.17% and 15.55%, respectively. Main artichoke heads and those at the advanced development stage presented the lowest phenolic concentration as well as the lowest PPO activity; therefore, they exhibit the lowest browning process, which could increase their use in ready-to-eat products at market.
Lemon postharvest losses are mainly due to the presence of fungal diseases. Current postharvest decay strategies rely on synthetic chemical fungicides; however, consumers are demanding that fruit is free of any chemical residue. The use of new natural alternatives, including essential oils, is emerging due to their potential antimicrobial activity. Therefore, the aim of this work is the elucidation of the effect of carvacrol, eugenol, and thymol, individually and in combination, applied in preharvest. Three different concentrations (100, 500, and 1000 µL/mL) of carvacrol, eugenol, and thymol were individually applied and in combination in ‘Fino’ and ‘Verna’ lemon cultivars. The fungal incidence (mainly Penicillium digitatum and P. italicum) was evaluated weekly for 35 days. Moreover, the main different quality parameters (weight loss, firmness, colour, total soluble solids, titratable acidity, and total phenolic content) of lemons were evaluated at harvest and after 35 days of cold storage. The results showed that carvacrol at the lowest concentration (100 µL/L) provided the lowest fungal incidence with a non-negative effect on the lemon quality parameters during storage, while the highest concentrations and the combination of essential oils resulted in the opposite effect. Therefore, carvacrol applied at 100 µL/L in preharvest could be an eco-friendly alternative to the current fungicides to control lemon decay, while maintaining their optimal quality.
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