Objectives: In this article, we aim to compare the contamination rates between neckties physicians in a large Brazilian hospital to those of lawyers. We also intend to compare the contamination rates between neckties and shirts from those two groups. We believe that neckties often serve as a fomite, carrying potentially harmful bacteria and, directly or indirectly, contaminate patients. Methods: We collected swab samples from doctors´ neckties and shirts at a large teaching hospital in São Paulo city center and assessed their degree of contamination. The findings were compared against necktie and shirt swab samples taken from law students (control group) of a University in Sao Paulo city center. The contamination levels of both fomites were assessed, together with the susceptibility profile of bacteria not from normal microbiota. Results: Contamination of doctors´ neck ties was greater than that of law students´ neckties (p<0.05), where pathogenic bacteria were detected in both groups. No statistically significant difference in contamination of shirts was found between the groups. Bacteria that make up normal human microbiota (Staphylococcus epidermidis, Staphylococcus aureus, Micrococcus sp, and Bacillus subtilis) were the most prevalent. Conclusions: In this study, both pathogenic bacteria and normal microbiota bacteria were found, highlighting the susceptibility of neck ties of doctors and law students to bacterial contamination. These garments can act as a vehicle for spreading antibiotic-resistant bacteria in the community.Keywords: Microbiology, Cross Infection, Fomites, Clotings, Humans RESUMOIntrodução: Diversos objetos podem agir como fômites para a contaminação bacteriana. Jalecos, aventais e camisas são normalmente higienizados, enquanto gravatas não. Estas poderiam transportar microorganismos capazes de colonizar outras áreas e, direta ou indiretamente, contaminar indivíduos debilitados. Assim, mesmo médicos que realizam higienização adequada das mãos podem se recontaminar pelo contato com gravatas. Materiais e métodos: Coletamos amostras com swabs das gravatas e camisas dos médicos de um hospital escola de grande porte no centro de São Paulo e avaliamos seu grau de contaminação. Comparamos os achados a um grupo controle com swabs de gravatas e camisas de estudantes de direito de uma universidade no centro de São Paulo. avaliamos as possibilidades da contaminação das foram avaliadas para ambos os fômites, além do perfil de sensibilidade das bactérias que não pertencem à microbiota normal. Resultados: A contaminação das gravatas de médicos foi superior à de advogados (p<0,05), evidenciando bactérias patogênicas em ambos os grupos. Não houve diferença estatisticamente significante na contaminação de camisas entre os grupos. Bactérias que compõem a microbiota normal humana foram mais frequentes (Staphylococcus epidermidis, Staphyococcus aureus, Micrococcus sp, bacilo Subtilis). Discussão: Neste estudo, encontramos bactérias patogênicas e bactérias da microbiota normal, evidenciando a susceptibilidade das gravatas de médicos e estudantes de medicina à contaminação bacteriana, podendo atuar na comunidade como meio de transporte para bactérias resistentes aos antibióticos.Descritores: Microbiologia, Infecção Hospitalar, Fômites, Roupas, Humanos
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