A clinical form of sepsis induced by cecal ligation and puncture (CLP) was caused in order to monitor the concentration of enzymes (alanine aminotransferase -ALT, aspartate aminotransferase -AST, lactate dehydrogenase -LDH, amylase and creatine kinase -CK) in the rat blood. Experiments were performed on male Wistar rats, weighing on average 215±25 g. The rats were divided into four groups. In the first three groups (n=28 per group), sepsis was induced by pure culture of Escherichia coli (EC) or Staphylococcus aureus (SA) and mixed culture (MK) of caecum, while the fourth group included 20 control rats who underwent an abdominal incision. Blood was taken in time intervals of 12, 24, 72 and 120 hours.During the experimental protocol, we identified significant changes of all monitored enzymes in the serum of infected rats. After a period of 12 hours there was a significant increase in ALT (all rats with sepsis), AST and LDH (rats in the MK group) levels, while a decrease was noted in the concentration of amylase (EC, SA). Similarly, 24 hours after the CLP procedure, a significant decrease of amylase (MK) and AST (SA) was recorded, while serum LDH level varied significantly from elevated (EC, SA) to reduced (MC) values. Finally, at the time intervals of 72 and 120 hours the concentration of nearly all monitored enzymes has shown a decline, while significance was noted in lowering of ALT (MK), AST (SA, EC) and amylase (SA) levels. Statistical significance could not be observed in the change of CK levels at any of examined time points.

The usage of microbiological screening tests is widespread in control of presence of antimicrobial drug residues in meat samples. Screening tests must be capable to detect antimicrobial drug residue of interest and detection limits must comply with MRL (Maximum Residue Limit). The aim of this study was to examine the performance of a microbiological screening test with E. coli as test microorganism: capability of detecting enrofloxacina and it's main metabolite ciprofloxacine at MRL levels in both fortified and incurred chicken tissue samples. Detection limits of microbiological screening test with E. coli was 50 ng/g for enrofloxacin and 25 ng/g for ciprofloxacin. Screening test had positive results in all samples of fortified and incurred meat with residue concentrations above MRL level. The results of this examinations shows that microbiological screening test with E. coli, as simple and cost effective test, is capable to detect enrofloxacine and it's metabolite ciprofloxacine in treated poultry at MRL level ie test is capable to detect unsafe poultry meat.

Canine dirofilariasis is a parasitic disease. The causative agent is Dirofilaria immitis or Dirofilaria repens. It may appear as skin or as a heart disease. The heart form of the disease is often named "heartworm" disease, because the adult parasites live in the heart. In the last five years, several cases of dirofilariasis have been reported (Dimitrijević, 1999; Savić-Jevđenić et al., 2004). At the beginning dirofilarias was discovered only as a by-the-way finding during autopsy, but last two years there were more requests for examination of dirofilariosis in alive dogs. Clinical symptoms in dogs with dirofilariosis are occasional coughing, apathy, breathing difficulties, heart failure, progressive loss of weight and rapid fatigue. The variety of symptoms depends on the intensity of infection, and the symptoms depend on the activity of a dog. For the diagnostics of dirofilariosis, the modified Knott's test or another serology methods may be used. In our research we used 45 blood samples, taken from the dogs in the region where dirofilariosis was detected mostly through autopsy, but also from the dogs with clinical symptoms in which cases the owners or veterinarians had sent the samples for analysis. The modified Knott's test was used for proving microfilaria in full blood and ELISA test was applied for detection of D. immitis antigen. Out of 35 examined dogs with no clinical symptoms, in 4 dogs the presence of antigene Dirofilaria immitis was detected in blood sera, which is 11% of the examined dogs. The examination of 10 dogs with clinical symptoms (coughing, difficult breathing, apathy and rapid fatigue) showed the presence of microfiliaria in circulation (ELISA test) and the presence of antigen D. immitis in 8 dogs. Therapy was successful in two cases.

The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in “Zasavica”, blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test).