One of the factors determining success in tomato production is plant nutrition. The diagnosis and recommendation integrated system (DRIS) is a tool used to obtain fertilizer recommendations. The objective of this study was to determine DRIS indices using petiole sap analysis (PSA) and compare them with those obtained using leaf analysis in two tomato cultivars grown under greenhouse conditions. A complete randomized design with eight treatments and 60 plants per experimental unit was used. To determine whether employing PSA with the DRIS methodology can provide a reliable diagnosis of fertilization, doses of some elements, such as N, P, or K, were reduced by 30% and supplied to two tomato cultivars, namely, Cid and Moctezuma. The indices for PSA-DRIS and DRIS were estimated. R2 values greater than 0.84 were obtained for the relationships between the leaf concentrations and petiole extract (PE) concentrations of some elements in both tomato cultivars. The PSA-DRIS method detected 62.5% of the cases of deficiency identified via DRIS. The PSA-DRIS method is a low-cost way to rapidly fertilize recommendations in tomato.