No abstract

The recombinant monoclonal antibody capture step represents the current bottleneck in downstream processing. Protein A resins are diffusion-limited chromatography materials which require low flow rates to achieve a binding capacity above 30 g L−1 with the result of low productivity. Here, we present a novel chromatography membrane combining superior binding capacities with high flow rates for high productivity while achieving comparable product quality as state-of-the-art protein A resins. Further, we demonstrate full scalability of this convecdiff technology with experimental data demonstrating suitability for bioprocessing at different scales. This technology results in more than 10-fold higher productivity compared to Protein A resins, which is maintained during scale up. We demonstrate the influence of residence times, feed titers and the cleaning regime on productivity and indicate optimal utilization of the convecdiff membrane based on feed titer availability. The underlying high productivity and short cycle times of this material enable the purification of monoclonal antibodies with 10-times less chromatography material used per batch and utilization of the membrane within one batch. Provided in disposable consumables, this novel technology will remove column handling in bioprocesses and resin re-use over multiple batches.

Our study aimed at the development of an effective method for citric acid production from glucose by use of the yeast Yarrowia lipolytica. The new method included an automated bioprocess control using a glucose biosensor. Several fermentation methodologies including batch, fed‐batch, repeated batch and repeated fed‐batch cultivation were tested. The best results were achieved during repeated fed‐batch cultivation: Within 3 days of cycle duration, approximately 100 g/L citric acid were produced. The yields reached values between 0.51 and 0.65 g/g and the selectivity of the bioprocess for citric acid was as high as 94%. Due to the elongation of the production phase of the bioprocess with growth‐decoupled citric acid production, and by operating the fermentation in cycles, an increase in citric acid production of 32% was achieved compared with simple batch fermentation.

Volumen XXIX, Fasciculus v (1946). 1267 calcium est beaucoup plus forte en presence de glycerophosphates que de phosphates. I1 en decode que la mise en libertk d'ions phosphoriques au niveau des territoires ou Bvolue la calcification provoque vers ceux-ci un veritable drainage des ions calcium presents dans les humeurs. Dks lors le r61e physiologique de l'enzyme apparait comme celui d'un agent de concentration locale en ions phosphoriques dans les os, concentration g r h e a laquelle s'opbre un appel des ions calcium des humeurs et, secondairement, la precipitation d'un phosphate de calcium insoluble, regie par le produit de solubilitk de ces ions.Ce rapide expose montre la diversite des domaines auxquels s'6tend la biochimie des phosphatases et les nombreux problkmes que pose son etude. La description des systbmes phosphatasiques naturels est aujourd'hui trks avancke, mais la connaissance des phosphatases en tant que molecules chimiquement dhfinies est B peine Bbauchde.C'est dire quelle importance rev6t la preparation de phosphatases pures, qui doit &re le plus immediat des buts qu'il convient de chercher