No abstract

The genesis of lipoleiomyoma has not been explained yet. Immunohistochemical examinations were performed on 17 lipoleiomyomas in women aged 43-82 (mean age: 51 ±9 years). Four types of myomas were distinguished: 1) pure leiomyoma, 2) fibroleiomyoma, 3) hyalinizing leiomyoma, 4) strongly hyalinized myoma, along with three degrees of progression of adipocytic metaplasia: 1) up to 25% of lipocytes, 2) up to 50% of lipocytes, and 3) over 50% of lipocytes in the analyzed sample, along with three degrees of progression of adipocytic metaplasia: 1) up to 25% of lipocytes, 2) up to 50% of lipocytes, and 3) over 50% of lipocytes in the analyzed sample. A positive correlation was found between the age of women and rate of development of metaplasia (r = 0.51, p = 0.035) as well as with activity of the estrogen receptor in the primary tumor (r = 0.53, p = 0.03). New mucous perivascular tissue was reported among 11.8% of patients and on this basis lipocytes were formed. The appearance of subendothelial granular cells of large blood vessels with a positive reaction for smooth muscle actin (SMA) and CD68 was reported in 17.7%. Results of immunohistochemical research seem to confirm that lipocytes de novo come from the primal pluripotent cells of the tumor stroma and not from the fatty degeneration of myocytes.

consent from study participants. The research was conducted in accordance with the Helsinki Declaration.Patients Blood samples were drawn from 23 healthy individuals (age range, 23-55 years; 11 men and 12 women), 13 disease controls (3 patients with oncocytoma, 2 with papillary carcinoma, 5 chromophobe renal cell carcinoma, and 3 with unclassified RCC; age range, 36-76 years), and 20 patients with ccRCC (age range, 48-89 years; 11 men and 9 women). Fresh renal tumor samples were obtained after partial or radical nephrectomy. This study also utilized 2 formalin -fixed paraffin -embedded (FFPE) prostate cancer samples, one from a patient with ccRCC whose renal tumor was positive for PIK3CA alteration (COSM754), and one from a patient with ccRCC whose kidney tumor was negative for PIK3CA alteration (a negative control for target sequencing).Targeted sequencing of cancer -associated genes and data analysis The genomic DNA was purified from blood, and tumor specimens using Pure-Link genomic DNA mini kit (Thermo Fisher Scientific, Waltham, Massachusetts, United States). The quality of DNA was assessed on agarose gel stained with ethidium bromide, and the quantity of DNA was measured using the Qubit 2.0 fluorometer (Thermo Fisher Scientific). The mutational status of "hotspot" regions (encompassing 2856 COSMIC mutations) in 50 cancer -related genes (ABL1,