Objective: The work is aimed to evaluate the health beneficial properties of (Beta vulgaris) Beetroot. Beetroot ranks among the 10 most powerful vegetable as a natural antioxidant and has a potential source of natural food colourant. The present work is therefore organized to evaluate the Total Phenolic Content (TPC), Antioxidant activity and Antibacterial activity of the Ethanolic and Methanolic extracts of Beetroot. Methods:In the present work the Total Phenolic Content was determined by using Folin-Ciocalteu reagent method of the Ethanolic and Methanolic extracts of Beetroot (Beta vulgaris). The antioxidant scavenging activity of these extracts was determined by applying three different assay methods: (1) (1, 1-diphenyl-2-picryl hydrazyl) DPPH method, (2) Ferric thiocyanate (FTC) method and (3) Thiobarbituric acid (TBA). The antibacterial test was examined against gram positive (B. subtilis, S. aureus) and gram negative (E. coli, S. dysenteriae) bacterial strains. Results:In the present work the Methanolic extract showed greater TPC value 394.8 mg/g GAE than the Ethanolic extract 316.8 mg/g GAE. A correlation between antiradical capacities of the extracts with TPC value was clearly observed. The Methanolic extract was found to be most effective in all the methods. 50% scavenging power of the Methanolic and Ethanolic extracts were (0.129 mg/ml and 0.254 mg/ml) in DPPH method respectively. Moreover, in TBA and FTC method, both the extracts of Beetroot exhibited strong percentage inhibition ranging from 49%-62%. The results of the antibacterial test indicated that the Ethanolic and Methanolic extracts of Beetroot are significantly effective, both in Gram-negative (E. coli, S. dysenteriae) and in Gram-positive (B. subtilis, S. aureus) bacterium. Conclusion:Thus, from the above experimental observations, it can be clearly stated that the Beetroot is a promising source of natural antioxidant and antibacterial agent and definitely provides an alternative towards synthetic antioxidant because of its beneficial properties.

The present work is designed to evaluate the antioxidant, antibacterial activity and (TPC) total phenolic content of Tagetes erecta flowers extracts. Antioxidant potential of flowers extracts was examined by applying three different methods. Antibacterial test was determined against gram positive and gram negative bacterium. The ethyl acetate extract had the greater phenolic concentration of 48.2 mg/g GAE followed by methanol extract which was 42.5 mg/g GAE and hexane extract 36.4 mg/g GAE. The ethyl acetate extract was found to be most effective in all the antioxidant methods in correlation with the TPC value. Moreover, all the flowers extracts are significantly effective in both Gram negative and Gram positive bacterium. Tagetes erecta flowers are hopefully considered as a promising source of natural pigments with a scope towards the alternative therapy for treatment of various health problems.

The Total Phenolic Content (TPC), antioxidant and antibacterial activities of methanolic extract of Marigold flower (MF) (Tagetes erecta) and Beet root (BR) (Beta vulgaris) were examined. The present work reveals that MF contained greater amount of TPC (42.5 mg/g GAE) as compared to BR (39.4 mg/g GAE). Methanolic extract of MF exhibited excellent DPPH free radical scavenging power (IC 0.0716 mg/mL) and reducing power at 1 mg/mL concentration. Similar results have been obtained in FTC and TBA method. The results of antibacterial test indicated that the methanolic extract of MF and BR is significantly effective against both type of Gram-negative (Escherichia coli, Shigella dysenteriae) and Gram-positive (Bacillus subtilis, Staphylococcus aureus) bacterial strains. Therefore, the present study suggests that the Marigold and BR are promising source of herbal medicinal products with noteworthy antioxidant and antibacterial activity.

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