Attachment of the cells of some bacteria, yeasts, and micromycetes to various surfaces (catheters, dentures, plastic, polyvinyl chloride, tiles, and steel) treated with the surfactants from Acinetobacter calcoace ticus IMB B 7241, Rhodococcus erythropolis IMB Ac 5017, and Nocardia vaccinii IMB B 7405 was studied. Adhesion of microorganisms to all the studied surfaces depended on the surfactant concentration and purity, kind of surface, and the test culture. Treatment with the surfactants from N. vaccinii IMB B 7405 (0.005-0.05 mg/mL), A. calcoaceticus IMB B 7241 (0.003-0.036 mg/mL), and R. erythropolis IMB Ac 5017 (0.03-0.12 mg/mL) resulted in adhesion decreased respectively by 35-75, 60-75, and 25-90% for bacteria (Escherichia coli IEM 1, Bacillus subtilis BT 2, etc.), by 80-85, 55−90, and 15-60% for yeasts Candida albi cans D 6, and by 40-50, 35-35, and 10-20% for micromycetes (Aspergillus niger P 3 and Fusarium cul morum T 7).

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Intensification of the surfactant synthesis by Rhodococcus erythropolis IMV Ac-5017 on different substrates, including industrial waste, as well as the use of surfactant preparations for oil degradation were studied. It was established that the addition of fumarate (0.2 %) and citrate (0.1 %) into the medium with ethanol, n-hexadecane, or glycerol (1-2 %) was accompanied by an increase of conditional surfactant concentration by 1.5-1.7 times compared to the indexes in the medium without organic acids. The intensification of surfactant synthesis in the presence of fumarate and citrate is caused by the increased activity of isocitrate lyase (by 1.2-15-fold) and enzymes of the surfactant biosynthesis (by 2-4.8-fold) compared to their activity in the medium without precursors. The possibility of surfactant synthesis intensification (by 3-4-fold) while cultivating of R. erythropolis IMV Ac-5017 in the medium with oil containing substrates (2 %) and glucose (0.1 %) was shown. The introduction of 0.01 mM Cu(2+) in the exponential growth phase of strain IMV Ac-5017 in the medium with ethanol accompanied by the increasing conditional surfactant concentration by 1.9 times. The highly efficient remediation (92-95 %) of oil (2-2.6 g/L) and Cu(2+) polluted water after treatment with surfactant preparations (native cultural liquid) at low concentrations (5 %) was determined.

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