Many surveys contain sets of questions (e.g., batteries), in which the same phrase, such as a reference period or a set of response categories, applies across the set. When formatting questions for interviewer administration, question writers often enclose these repeated phrases in parentheses to signal that interviewers have the option of reading the phrase. Little research, however, examines what impact this practice has on data quality. We explore whether the presence and use of parenthetical statements is associated with indicators of processing problems for both interviewers and respondents, including the interviewer's ability to read the question exactly as worded, and the respondent's ability to answer the question without displaying problems answering (e.g., expressing uncertainty).Data are from questions about physical and mental health from 355 digitally recorded, transcribed, and interaction-coded telephone interviews. We implement a mixed-effects model with crossed random effects and nested and crossed fixed effects. The models also control for some respondent and interviewer characteristics. Findings indicate respondents are less likely to exhibit a problem when parentheticals are read, but reading the parentheticals increase the odds (marginally significant) that interviewers will make a reading error.

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Human pluripotent stem cell (hPSC)-derived retinal organoids (ROs) can efficiently and reproducibly generate retinal neurons that have potential for use in cell replacement strategies [Capowski et al. , Development  146, dev171686 (2019)]. The ability of these lab-grown retinal neurons to form new synaptic connections after dissociation from ROs is key to building confidence in their capacity to restore visual function. However, direct evidence of reestablishment of retinal neuron connectivity via synaptic tracing has not been reported to date. The present study employs an in vitro, rabies virus-based, monosynaptic retrograde tracing assay [Wickersham et al., Neuron  53, 639–647 (2007); Sun et al., Mol. Neurodegener.  14, 8 (2019)] to identify de novo synaptic connections among early retinal cell types following RO dissociation. A reproducible, high-throughput approach for labeling and quantifying traced retinal cell types was developed. Photoreceptors and retinal ganglion cells—the primary neurons of interest for retinal cell replacement—were the two major contributing populations among the traced presynaptic cells. This system provides a platform for assessing synaptic connections in cultured retinal neurons and sets the stage for future cell replacement studies aimed at characterizing or enhancing synaptogenesis. Used in this manner, in vitro synaptic tracing is envisioned to complement traditional preclinical animal model testing, which is limited by evolutionary incompatibilities in synaptic machinery inherent to human xenografts.