A pharmacological approach to inhibition of cyclin-dependent kinases 4 and 6 (Cdk4/6) using highly selective small molecule inhibitors has the potential to provide novel cancer therapies for clinical use. Achieving high levels of selectivity for Cdk4/6, versus other ATP-dependent kinases, presents a significant challenge. The pyrido[2,3-d]pyrimidin-7-one template provides an effective platform for the inhibition of a broad cross-section of kinases, including Cdks. It is now demonstrated that the modification of pyrido[2,3-d]pyrimidin-7-ones to include a 2-aminopyridine side chain at the C2-position provides inhibitors with exquisite selectivity for Cdk4/6 in vitro. This selectivity profile is recapitulated in cells where the most selective inhibitors create a G(1) block at concentrations up to 100-fold the IC(50) for cell proliferation. On the basis of its selectivity profile and pharmacokinetic profile, compound 43 (PD 0332991) was identified as a drug candidate for the treatment of cancer.
The identification of 8-ethyl-2-phenylamino-8H-pyrido[2, 3-d]pyrimidin-7-one (1) as an inhibitor of Cdk4 led to the initiation of a program to evaluate related pyrido[2, 3-d]pyrimidin-7-ones for inhibition of cyclin-dependent kinases (Cdks). Analysis of more than 60 analogues has identified some clear SAR trends that may be exploited in the design of more potent Cdk inhibitors. The most potent Cdk4 inhibitors reported in this study inhibit Cdk4 with IC(50) = 0.004 microM ([ATP] = 25 microM). X-ray crystallographic analysis of representative compounds bound to the related kinase, Cdk2, reveals that they occupy the ATP binding site. Modest selectivity between Cdks is exhibited by some compounds, and Cdk4-selective inhibitors block pRb(+) cells in the G(1)-phase of the cell division cycle.
5,6-Dihydrothymid-5-yl (4) is generated via Norrish
type I cleavage of isopropyl ketone 7. Ketone 7
was
site specifically incorporated into chemically synthesized
polythymidylates and an oligonucleotide containing all
four native deoxyribonucleotides. No damage is induced in
oligonucleotides containing 7 upon photolysis
under
anaerobic conditions. In the presence of O2, strand
breaks and alkaline labile lesions are formed at the original
site
of 7, and at nucleotides adjacent to the 5‘-phosphate of
7. Kinetic isotope effect experiments reveal that
direct
strand scission at the thymidine adjacent to the 5‘-phosphate of
4 arises from C1‘ hydrogen atom abstraction.
The
observed KIE (∼3.9) is attributed to hydrogen atom abstraction from
C1‘ by the peroxyl radical 35 derived from
4.
Enzymatic end group analysis and measurement of free base release
are consistent with a process involving C1‘
hydrogen atom abstraction. Cleavage experiments carried out in the
presence of t-BuOH (1.05 M) and NaN3
(10
mM) indicate that damage does not result from hydroxyl radical, but
that 1O2 is responsible for a significant
amount
of the observed strand damage.
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