Exposure to biological agents and dusts occurs in homes and occupational environments and it is known to cause adverse health effects. There is limited information concerning the occupational exposure levels of airborne biohazard during wood processing, but this exposure is associated with a range of adverse health effects. Control of exposure to microbiological hazards and dust in woodworking is not easy. In fact, various types of wood are commonly used and they generate complex mixtures of dusts and biological agents with various health risks. The aim of this study was to investigate the concentration of dust, bacteria, and endotoxins encountered in six different wood factories. These people were exposed to between 0.05 and 12.00 mg inhalable dust m(-3) and between 0.40 and 6.93 ng inhalable endotoxins m(-3). Total bacteria concentrations in the air of the factories examined were within a range of 130-2000 CFU m(-3), the value of Gram negative was within a 0-164 CFU m(-3), and the concentration of Gram positive was within 1-104 CFU m(-3). In conclusion, people working in wood factories may be exposed to high levels of inhalable dust and endotoxins.

The peculiar characteristics of the greenhouses as confined spaces, microclimate and poor air exchange with the outside environment, encourage the development of a large number of biological agents. Endotoxin, is probably a major causative agent of occupational health problems. The objective of this study was to measure the concentrations of airborne endotoxin in greenhouses with different cultures. The influence of microclimate was studied in correlation with endotoxin levels and type of cultured vegetables. The data indicate that workers employed greenhouses are exposed to low levels of inhalable endotoxins; endotoxin concentrations do not correlate with the temperature and relative humidity values. A strong correlation between the leaf size and endotoxin concentration was observed. The mean concentration of endotoxins in the air of greenhouses is relatively low, however, there could be peaks of exposure during harvesting and eradication of broadleaf plants.

Dermal exposure of workers to pesticides can be assessed using patches, placed on the workers’ clothes or used to wipe off the substance from the skin. Since there are no official indications of the materials to be used for patch sampling, a wide range of materials is suggested in the scientific literature. This paper reports a laboratory study on the affinity of four different pesticides widely used in southern Italy with five patch matrices. Imidacloprid, Hexythiazox, Boscalid and Myclobutanil were tested with cotton and gauze sheets, polyethylene tissue and two different grades of cellulose papers. An aerosol machine was used to nebulize the substance on the patches in a closed system, simulating the conditions of use on the workers’ clothes. The recovery of the analytes from the media was evaluated, by spiking the patches with a known amount of each active substance and testing their performances as skin wipes. Samples were extracted and analyzed in gas chromatography with an electron capture detector. The recovery from the spiked patches was 89–96% for all pesticides, while the test recoveries were very different. Results showed a higher affinity with Imidacloprid and Hexythiazox for gauze, with Myclobutanil for cotton and with Boscalid for paper filters (W41).