Nurses’ life satisfaction (LS) predicts their health and the level of care they provide to patients, thus policies for promoting quality of nurses’ work require actions to increase their LS. The aim of this study was to examine relations between LS and two levels of personality (traits and values) among Polish nurses, including joint effects of traits and values in a model integrating all variables to check whether meta-values can mediate trait–LS relationships. Nurses (N = 155) aged 23–64 completed the NEO-FFI, Satisfaction with Life Scale, and PVQ40. LS correlated with all traits, with openness higher than usual (0.34), and positively associated with meta-values: openness to change (0.23), self-transcendence (0.30), and (‘unhealthy’) conservation (0.19). Trait–value consistency was insufficient to explain some trait–value associations. In the SEM analysis, 23.3% of LS variance was explained. LS was related directly to neuroticism negatively and positively not only to extraversion, but also to openness, and self-transcendence meta-value (that increased value-environment congruence), and indirectly positively (through self-transcendence) to openness, agreeableness, conscientiousness, and even neuroticism. These results indicate that promoting nurses’ health and quality of work by enhancing their LS requires supporting and increasing their identification with self-transcendence values and encourage research on factors that can increase it.