The cuscus is an Australian animal (marsupial) which belongs to the Phalangeridae family and its distribution is limited in eastern Indonesia, Australia and Papua New Guinea. Through IUCN data, cuscus is categorized as endangered species, in CITES it is classified as Appendix II. The population of cuscus is decreasing due to the threat of deforestation, many are hunted for consumption, and are traded illegally. To overcome the above problems, it is necessary to conduct a comprehensive study of the study of cuscus genotypes in the hope that they can assist in future wildlife conservation efforts. The purpose of this study was to molecularly examine the types of living cuscus in captivity in Lumoli Village, West Seram Regency, Maluku. This research begins with the stages of DNA isolation through cuscus tissue. DNA isolation products were amplified in the ATP 8 gene region by the PCR method, sequenced. Data was analyzed using MEGA program version 5.1. The PCR reaction produces 681 bp of product. The results of the analysis obtained 85 different nucleotide sites. The nucleotide sequence of the ATP 8 gene was analyzed using kimura 2 parameters. The construction of the filogram using the neighbor joining method with a bootstrap value 1000 times based on the ATP 8 gene sequence shows the kinship between the four types of cuscus, which produces two branches of Phalanger and Spilocuscus, namely brown cuscus related to white cuscus and related cuscus with spotted cuskus.

Cuscus is an Australian animal (marsupial) whose distribution is limited in Indonesia and its population is declining due to threats hunted for consumption, and traded illegally. To overcome the above problems it is necessary to conduct a comprehensive study of the type of feed in the hope of helping the conservation of these animals, especially in captivity. The purpose of this research is to study the types of plants as cuscus that live in captivity of Lumoli Village, West Seram District. This research begins with the observation of the type of kursus that lives in captivity of Lumoli Village. Study of feed type, depiction, and analysis. The results showed 44 species of plants in the village of Lumoli West Seram District, Maluku can be used as cuscus feed in captivity. Very good plant part as Source of Feed is young leaf of 28 species of plant (63,63%), 25 species (56,81%) are fruits, 4 species (9,09%) are flowers, 3 species (6,81 %) is a young shoot. The level of palatability of the cuscus indicates that the brown cuscus and white cuscus correspond to 43 plant species (97.72%) and can not be done 1 plant species (2.27%) (water apple), while the spotted cuscus and the gray magic cuscus 43 (97.72 %) plant types and unlike 1 plant species (2.27%) (Tomi-tomi). The proximate test results showed the highest ash content was available in the gray cuscus (18.95%) and the lowest in brown couscous (11.41%), the highest crude protein content in the gray cuscus (28.01%) and the lowest in white cuscus (23, 64%), crude fat content in cuscus totol (4.92%) and lowest in gray cuscus (2.93%), crude fiber content in gray cuscus (27.99%) and lowest in brown cuscus (21, 68% ). Can be concluded there are 44 types of forest plants that can be consumed either in the form of young leaves, fruit, flowers and young shoots. Further research is needed on additional types of feed for the cuscus in captivity.