This paper is focused on a design of a high-voltage (HV) generator, which is proposed for a high-frequency irreversible electroporation (H-FIRE). The generator produces bursts of bipolar symmetrical pulses. Most HV sources used for cell electroporation are based on a controlled discharge of a capacitor into a resistive load. This solution is very simple, but it is associated with a certain risk of an uncontrolled discharge of the capacitor. We present a different type of the generator, where a DC-AC inverter with pulse transformer is used and where the mentioned risk is eliminated. Our generator is able to deliver bursts with variable length from 50 to 150 μs and a gap between bursts can be set from 0.5 to 1.5 s. Pulse frequency can be varied from 65 to 470 kHz and the output voltage is controlled in two ranges from 0 to 1.3 kV or from 0 to 2.5 kV. Results are presented with resistive load and with tissue impedance load.

This paper discusses an application of different waveforms during a cell electroporation process. Latest scientific papers emphasize advantages of asymmetrical waveform applied to tissue. Higher effect of the electroporation process is reached and muscle contractions are less significant. Most highvoltage generators are based on a controlled discharge of storage capacitors. We present a different solution of the generator, where a power part contains a pulse transformer.

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This contribution refers about a design and development of a regulated DC-DC converter with pulse transformer, which is powered from a battery or an on-board car network. This converter with adjustable output voltage and current value can supply many appliances. The design of pulse transformer, secondary rectifier, output filter, switching transistors, and control circuits is included.

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