We describe the use of immobilized deoxyribonucleic acid (DNA) in a silica matrix as a biorecognition agent for the detection of albendazole sulfoxide (ASU), the primary metabolite of albendazole and a suspected teratogenic and embryotoxic agent. The biomaterial (DNA-containing gel) was synthesized by physical entrapment of salmon sperm in an inorganic silicate matrix by the sol-gel method. Functionality of the DNA-containing gel was evaluated by comparative offline frontal chromatography followed by HPLC analysis of ASU and caffeine (CAF, control) using DNA-containing gel and DNA-free gel. The DNA-containing gel showed relatively high specific retention for ASU, while CAF showed no retention using frontal analysis. We anticipate that the DNA-containing gel can be implemented to identify the interactions of DNA with other active pharmaceutical ingredients (APIs) and their metabolites in a readily available, sensitive and selective frontal chromatography experiment.

Antioxidants are indispensable molecules that help prevent biomolecule oxidation. By fighting the production of free radicals, antioxidants prevent different ageing-associated diseases. In this work, the method of 2,2-diphenyl-2-picrilhydracil (DPPH) is adapted to determine antioxidants in different cosmetic, nutritional, and biological samples using spectrophotometric and electrochemical techniques. Antioxidant molecules of Trolox (Tx) and gallic acid are used as a standard, whereas spectroscopic and electrochemical properties of DPPH free radical are used to determine total antioxidant capacity (meq Tx/mL) in different commercial products and biological samples. Oxidation-reduction properties of DPPH at a carbon glass electrode allow applying cyclic voltammetry and chronoamperometry to construct amperometric calibration curves

AI CONACYT por el apoyo económico recibido para realizar los estudios de Maestría (No de becario 11 1635). AI Área de Electroquímica, lugar en donde se aprende mucho de la vida.Ambiente en el que se comparte trabajo, amistad, cariño, sin faltar el conocimiento científico.Agradezco el tiempo, los conocimientos y la disposición de los sinodales para revisar y dar sus aportaciones para mejorar el trabajo escrito; así como por el tiempo dedicado para asistir a la defensa oral de este trabajo.