Glucose electrodes were prepared by "wiring" quinoprotein glucose dehydrogenase, GDH (EC 1.1.99.17) to glassy carbon with an osmium complex containing redox-conducting epoxy network. Their current density at 70 mM glucose concentration reached 1.8 mA cm-2 when 15 #tg cm-2 of the enzyme having an activity of 250 units mg-1 was applied to the electrode. Under the same conditions, electrodes made with glucose oxidase (GOX) of similar activity (250 units mg-1) had a maximum current density of 0.88 mA cm-2. The maximum current density was reached with 8 % GDH In the redox polymer film. The current density was almost flat through the 6.3-8.8 pH range and was not altered when the solution was either aerated or argon purged. It decreased at 25 °C to half Its Initial value In 8 h.
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