Citropsis articulata, also locally known as the Omuboro is a popular plant in Central and Western Uganda for its claimed aphrodisiac properties. The plant naturally grows in forests and locals uproot it to obtain the roots for treatment of erectile dysfunctions. The root of this plant has been cherished as one of the most valuable herbal Viagra by most rural communities in Uganda. 1 The fast disappearance of these culturally important medicinal plants due to poor utilization practices certainly pose major threat towards a looming extinction. Conservationists in the country are concerned that demand for the roots of the plant threatens the survival of this species' genetic diversity in conservation areas like Mabira forest. 2 The roots are either chewed while fresh/dried or pounded into powder form and then mixed with water to form a concoction. Other plants like Warburgia ugandenesis, Cola acuminata, Prunus americana and Ekebergia capensis have been used in the ABSTRACT Background: Erectile dysfunction is the inability to sustain erection of the penis firm enough for sexual intercourse in males. Citropsis articulata is used locally by communities in Uganda for the management of erectile dysfunction. The current study evaluated the effect of ethanolic leaf extract of C. articulata on the serum level of testosterone and mounting frequency in Male albino rats. Methods: The study animals were divided into four groups and the extract groups dosed daily orally with 400 mg/kg and 600 mg/kg of extract for three weeks. Sildenafil citrate (1mg/kg) and distilled water (10 ml/kg) were used as positive and negative control respectively. The mounting frequencies of the males exposed to estrus induced females rats on day (7 and 21), testosterone levels (day 21) and phytochemicals present in the extract were determined. Results: At 400 mg/kg and 600 mg/kg, both testosterone level and mounting frequency increased significantly (p<0.05) by third week. The mean testosterone level at 600 mg/kg (2.678 ng/ml) of the extract was higher than that of group treated with 1mg/kg sildenafil citrate (2.18 ng/ml). The aphrodisiac activity of this extract may be associated with presence of saponins and basic alkaloids in the plant extract. Conclusions: Taken together, the crude leaf extracts of C. articulata could be used as a cheap alternative for the treatment of low libido due to testosterone deficiency. However, further studies are needed on the safety profile and the identification of the molecules responsible for the biological activity of the plant extract.