No abstract

To handle stricter safety regulations combined with increasing complexity and shorter development cycles, it is necessary to consider safety aspects starting from the early phases of design. This paper presents an approach to link methods of safety analysis and modeling (SysML). Even though SysML and MBSE are common in the early stages of system design, there is a lack of methods integrating model-based design activities and safety analyses. Existing approaches either focus on particular tasks or build models after conducting separate safety analyses. Our presented approach, tailored to early stages of system design, introduces a "Hazard Analysis" SysML profile accompanied by a procedure for its application within a model-based safety analysis. It provides a preliminary hazard analysis and facilitates the systematic identification of safety-critical functions and components.

No abstract

Integrins, as transmembrane heterodimeric receptors, have important functions in cell adhesion, migration, proliferation, survival apoptosis and signal transduction, in many physio- as well as pathophysiological settings. Characterisation of integrins and their ligand/antagonist binding is notoriously difficult, due to high integrin redundancy and ubiquity. Bypassing the intrinsic difficulties of cell-based integrin expression, purification and reconstitution, we present for the first time the synthesis of a heterodimeric integrin receptor and its assembly into a block-copolymeric membrane mimic. We present comprehensive data to demonstrate the synthesis of functionally active integrin αv β3, generated by in vitro membrane-assisted protein synthesis (iMAPS). This work represents the first step towards a robust and adaptable polymer-based platform for characterisation of integrin-ligand interactions.