Trametes versicolor is a white-rot fungus known as a producer of extracellular enzymes such as laccase, manganese-peroxidase, and lignin-peroxidase. The production of these enzymes requires detailed knowledge of the growth characteristics and physiology of the fungus. Submerged cultivations of T. versicolor on glucose, fructose, and sucrose as sole carbon sources were performed in shake flasks. Sucrose hydrolysis catalyzed by the whole cells of T. versicolor was considered as one-step enzymatic reaction described with Michaelis-Menten kinetics. Kinetic parameters of invertase-catalyzed sucrose hydrolysis were estimated (K (m) = 7.99 g dm(-3) and V (m) = 0.304 h(-1)). Monod model was used for description of kinetics of T. versicolor growth on glucose and fructose as sole carbon sources. Growth associated model parameters were estimated from the experimental results obtained by independent experiments (mu(G)(max) = 0.14 h(-1), K(G)(S) = 8.06 g dm(-3), mu(F)(max) = 0.37 h(-1) and K(F)(S) = 54.8 g dm(-3)). Developed mathematical model is in good agreement with the experimental results.

Extended AbstractDue to generation of large quantities of waste [1], waste management has grown to become one of the biggest challenges these days. From economic and social to environmental factors, waste management and waste disposal need better management system because problems are often connected with technically weak waste management system, insufficient financial resources and often limited cooperation from public [2]. In order to cope better with this situation, waste is divided into fractions. Organic fraction of municipal solid waste is one of the classifications made to better adjust approach for recycling of biodegradable waste material. In the attempt to switch from linear to circular economy, the most common way of disposal organic fraction of municipal solid waste in the landfills has become the least favourable solution [3]. As a right step towards recycling biodegradable organic fraction of municipal solid waste, as a part of circular economy, environmentally and economical acceptable, composting found its place in the waste management. Because of benefits such as sanitization of waste, cost effectiveness and conversion of waste to valuable product, composting is considered as reliable biodegradable waste treatment option [1].Composting is a natural aerobic degradation process conducted and catalyzed by microorganisms, carried out under controlled conditions. To improve composting process regarding enhanced biodegradation, acceleration, nutrient content and ratios, reduction of gas emissions, reduction of leachate generation, various additives could be used. Addition of biological agents, such as specific microbe population specifically applied through bioaugmentation, can affect composting process greatly toward faster and better conversion of biodegradable material in to useful products [4]. Many biodegradation studies have focused on survival of isolated bacteria to increase the bacteria population and subsequently enhance the efficiency of biodegradation [5].The primary aim of this study is to enhance and accelerate biodegradation of biowaste by composting process in closed reactor system. Biological waste from households, originating from gardens and yards, was designated as a substrate for reactor composting. To investigate the effect of microorganisms in the composting process, one bacterial isolate from biowaste compost was selected. Experiments were performed in two reactors with (R1) and without (R2) bioaugmentation during 6 days. Bacteria culture Sphingobacterium spritivorum has been isolated, identified and bioaugmented in reactor R2. Initial values for the experiments were respectively, volatile matter 58.27 %, moisture content 71 %, C/N ratio 14.47, and pH 7.46. In the reactors, on day 6 of composting process, conversions were 23.90 % and 38.22 % for R1 and R2 respectively. At the end of the experiments, emissions of CO2 and NH3 were 236.65 g/kgVM0 and 317.83 mg/ kgVM0 for R1, and 259.40 g/kgVM0 and 97.33 mg/ kgVM0 for R2. C/N ratios were 12.25 and 11.55 for R1 and R2 respectively. I...