The incidence of renal cell carcinoma (RCC) is increasing worldwide, and its prevalence is particularly high in some parts of Central Europe. Here we undertake whole-genome and transcriptome sequencing of clear cell RCC (ccRCC), the most common form of the disease, in patients from four different European countries with contrasting disease incidence to explore the underlying genomic architecture of RCC. Our findings support previous reports on frequent aberrations in the epigenetic machinery and PI3K/mTOR signalling, and uncover novel pathways and genes affected by recurrent mutations and abnormal transcriptome patterns including focal adhesion, components of extracellular matrix (ECM) and genes encoding FAT cadherins. Furthermore, a large majority of patients from Romania have an unexpected high frequency of A:T4T:A transversions, consistent with exposure to aristolochic acid (AA). These results show that the processes underlying ccRCC tumorigenesis may vary in different populations and suggest that AA may be an important ccRCC carcinogen in Romania, a finding with major public health implications.

Mass spectrometry (MS)-based quantitative proteomics experiments typically assay a subset of up to 60% of the ≈20 000 human protein coding genes. Computational methods for imputing the missing values using RNA expression data usually allow only for imputations of proteins measured in at least some of the samples. In silico methods for comprehensively estimating abundances across all proteins are still missing. Here, a novel method is proposed using deep learning to extrapolate the observed protein expression values in label-free MS experiments to all proteins, leveraging gene functional annotations and RNA measurements as key predictive attributes. This method is tested on four datasets, including human cell lines and human and mouse tissues. This method predicts the protein expression values with average R 2 scores between 0.46 and 0.54, which is significantly better than predictions based on correlations using the RNA expression data alone. Moreover, it is demonstrated that the derived models can be "transferred" across experiments and species. For instance, the model derived from human tissues gave a R 2 = 0.51 when applied to mouse tissue data. It is concluded that protein abundances generated in label-free MS experiments can be computationally predicted using functional annotated attributes and can be used to highlight aberrant protein abundance values.

Background: One of the crucial aspects of day-to-day laboratory information management is collection, storage and retrieval of information about research subjects and biomedical samples. An efficient link between sample data and experiment results is absolutely imperative for a successful outcome of a biomedical study. Currently available software solutions are largely limited to largescale, expensive commercial Laboratory Information Management Systems (LIMS). Acquiring such LIMS indeed can bring laboratory information management to a higher level, but often implies sufficient investment of time, effort and funds, which are not always available. There is a clear need for lightweight open source systems for patient and sample information management.

Various studies demonstrate that data on mobile phone use are useful when analysing problems in the fields of human activity or population dynamics, including tourism, transportation planning, public administration, etc. However, one of the biggest challenges is related to the restrictions contained in the General Data Protection Regulation that force the use of statistics about mobile operator client activities instead of allowing the analysis of mobile operator data. Therefore, a data analytics approach that does not involve information on the mobility of particular persons was developed, providing economically relevant data on aggregate mobility while protecting personal data. The activity data aggregation was conducted at 15-min intervals in the area of each cellular base station; "activity" is defined as the number of outgoing and incoming calls and sent and received text messages (short message service or SMS) and, in some instances, as the count of unique users. The case study examines all of Latvia's municipalities, analysing the economic activity level in each municipality in comparison to the mobile phone activity

We consider representing of natural numbers by expressions using 1's, addition, multiplication and parentheses. n denotes the minimum number of 1's in the expressions representing n. The logarithmic complexity n log is defined as n /log 3 n. The values of n log are located in the segment [3, 4.755], but almost nothing is known with certainty about the structure of this "spectrum" (are the values dense somewhere in the segment etc.). We establish a connection between this problem and another difficult problem: the seemingly "almost random" behaviour of digits in the base 3 representations of the numbers 2 n . We consider also representing of natural numbers by expressions that include subtraction, and the so-called P -algorithms -a family of "deterministic" algorithms for building representations of numbers.