Background: This study sought to assess the effect of Er,Cr:YSGG laser power on the surface roughness of lased radicular dentin using atomic force microscopy (AFM). Methods: Fifteen human radicular dentin samples were randomly divided into five groups: one control (G1, intact) and four experimental groups treated with 0.25W (G2), 0.5W (G3), 0.75W (G4) and 1W (G5) powers of Er,Cr:YSGG laser (2.78µm) at a repetition rate of 20 Hz, with a 600-µm-diameter sapphire tip. After irradiation, surface topography was analyzed by AFM using a Si probe in tapping mode. Quantitative information concerning the arithmetic average roughness (Ra) and quadratic mean roughness (Rq) was obtained from three 5×5µm areas of each sample. The data were analyzed using one-way ANOVA (P<0.05). Results: The Ra and Rq values increased in G2 and G5 and decreased in G3 and G4 groups compared to the control group. The maximum Ra and Rq values were noted in G5, which were significantly higher than the corresponding values in G3 (P<0.05). Conclusion: No direct correlation was found between Er,Cr:YSGG laser power and surface roughness of lased radicular dentin. Laser therapy with a mean power of 0.5W and 1W caused the lowest and highest surface roughness, respectively.