Development of ozone technology fish storage system (OTFIS) to maintain the quality of the fish has been done. The ozone technology fish storage system (OTFIS) can be applied in the fishing boat, fish storage in the Fish Auction Market, fish storage in the means transport for fish. In this research, we produced a prototype that was integrated by the fish container and ozone generator. We used Dielectric Barrier Discharge Plasma (DBDP) technology for ozone generator. The characterization of ozone generator has been carried out and showed that ozone generator can produce ozone concentration from 0.5 ppm to 12 ppm. The ozone concentration in the area is very suitable to be used in food technology. The ozone treatment was performed on red tilapia representing the white-fleshed fish with ozone concentrations from 0.5 ppm to 4.5 ppm. The results of this treatment showed that organoleptic and TVBN of fish were still appropriate with Indonesian National Standard (INS). Beside that, TPC testing has been done on the red tilapia showed that the higher the ozone concentration the smaller the remaining of bacteria colonies. Similarly, for a variable of time, the longer the exposure to ozone the less the remaining of colonies. The results of this study have been adapted to realize of OTFIS with a size of 50 cm x 50 cm x 100 cm (250 liters) and can accommodate as much as 100 kg of fish.

Research on ozone production by using silent plasma or Dielectric Barrier Discharge Plasma (DBDP) and implemented for microbial inactivation in rice has been done. The plasma reactor for ozone generating were reactor with multipoint-plane (MP-P) reactor and spiral-cylender (S-C) reactor configurations. The high voltage AC has been used with voltage up to 40 kV and maximum frequency 23 kHz. For implementation in rice, this research used a Randomized Block Design (RBD) with 5 treatments and 4 replications in each treatment. Exposure time was varied such as: 0, 30, 60, 90, and 120 minutes. Total bacteria and molds (fungi) has been detected by using Total Plate Count (TPC) methods with nutrient broth as media for bacteria and, we found that MP-P Reactor needed less voltage comparing with S-C Reactor for the same cocentration ozone. Ozone generated by DBDP can reduce the population of bacteria and fungi in rice. The exposure for 90 minutes can eliminate 58.41% population of bacteria, and 26.23% population of fungi. Through analysis of variance known that ozone treatment is significantly affect the total bacteria at significance level of 5% and 1% (P <0.05). However, treatment of fungi shown no significant effect, even at the 5% significance level (P> 0.05). Subtances of water, fat, and protein in rice was not change before and after ozone treatment.