BACKGROUND: Wound management is one of the significant health problems throughout the world. Medicinal plants have been used widely in wound management. Eurycoma longifolia Jack which is known as Tongkat Ali (TA) is a tropical medicinal plant in South East Asian countries. AIM: The aim of the study was to investigate the effect of (TA) hydrogel on wound contraction and re-epithelialization in excisional wound model in rats. METHODS: Twenty male Sprague Dawley rats were divided into four groups each group contained five rats (n = 5). Animal treatment groups are formed as: Untreated (−ve) control, Hydrocyn® aqua gel (+ve), vehicle hydrogel, and (TA) hydrogel. A full-thickness circular excisional wound was created on the dorsal back of each rat. The wounded area was measured and photographed on days 3, 6, 9, 12, 15, and 18 post wounding to determine the percentage of wound contraction and re-epithelialization. RESULTS: (TA) hydrogel showed significant increase in the percentage of wound contraction by 43.38% compared with the other groups (p = 0.032, p < 0.050) during the first interval (inflammatory phase). Although in the later healing stages (proliferative and remodeling) and re-epithelialization, our test group (TA) hydrogel did not show statistically difference with the other groups yet it was comparable to medically certified wound healing agent. CONCLUSION: (TA) hydrogel significantly accelerated the wound healing process during the early stage, the inflammatory stage. Whereas during the later healing stages and re-epithelialization, it showed almost the same effect of Hydrocyn® aqua gel.

INTRODUCTION: Medicinal plants are known for their positive impacts on wound healing by stimulating angiogenesis in the skin. Eurycoma longifolia Jack locally known as Tongkat Ali (TA), is a medicinal plant characterised by anti-inflammatory and antioxidant effects. MATERIALS AND METHODS: Excisional wound (15mm × 15mm) in diameter and (2mm) depth was created at the back of 20 male Sprague Dawley rats by incising the marked skin with sterilized surgical scalpel blade then excised the skin by surgical scissors and toothed forceps. The wounded rats were divided into 4 groups, each group contained 5 rats (n=5). Experimental groups were formed as follows: untreated (-ve) control, Hydrocyn® aqua gel (+ve) control, vehicle hydrogel, and TA hydrogel. All the treatments were applied twice daily for 5 days starting on the first day (wounding day). On Day 5 post-wounding, the granulation tissue was harvested from all groups and evaluated by immunohistochemistry assay for Vascular endothelial growth factor (VEGF) expression. RESULTS: VEGF expression in granulation tissue of rat’s skin treated with TA hydrogel and vehicle hydrogel increased significantly compared to that in the untreated (-ve) control group with p-values of 0.040 and 0.029, respectively. Although there was no significant difference in VEGF expression in granulation tissue of rat’s skin treated with the TA group, Hydrocyn® aqua gel (+ve) control, and vehicle hydrogel groups, our study group showed higher expression of VEGF. CONCLUSION: Our study showed that the topical application of TA hydrogel increased the VEGF expression in granulation tissue of rat’s skin, which is an essential growth factor for wound healing. Thus, there is great potential for TA hydrogel to be an effective wound-healing agent for managing cutaneous wounds.