The relative contribution of a theory-based intervention to modify high-risk behaviors in training programs is a major priority that remains an open question. Here, we tested whether the family-centered empowerment model used in the educational intervention was effective to modify high-risk behaviors of brucellosis via mother education. A quasi experimental study was conducted on 200 women presenting to healthcare practices in rural areas of Torbat-e Jam, Iran, from April 2020 to February 2021. Four rural areas were randomly assigned to the control and intervention groups. The intervention group received the training program, which included four 2-h sessions and consulting support via social network and messaging service. The control group did not receive any training. SPSS16 was implemented to test multiple statistical analyses. Our finding showed in the intervention group compared with the control group, knowledge, attitude, self-efficacy, self-esteem, and behavior outcomes were significantly changed (P < 0.001) across time during baseline through follow-up. Likewise, there are no differences (P > 0.05) in the change in construct of the family-centered empowerment model and risk behaviors in the control group from baseline to follow-up. Intervention based on a family-centered empowerment model is possible and very acceptable to modify high-risk behaviors of brucellosis by increasing an individual’s knowledge, changing attitude, and promoting self-efficacy and self-esteem.Trial registration: Iranian Registry of Clinical Trials (IRCT), IRCT20160619028529N12. Registration date: 24/03/2020.