E-learning and self-regulation are kinds of autonomous learning. Some researchers have long been interested in E-learning and self-regulation. The purpose of this article is to study the EFL learnersʹ perception of using E-learning and self-regulation in English classrooms. A quantitative research method was used for 360 intermediate, advanced learners and 34 teachers. Cluster sampling and convenience sampling method in order used for EFL learners and the teachers. Data gathered for six weeks in Zanjan English language institutes. The questionnaires were about advantages and disadvantages of e-learning and participants' perception on self-regulation capabilities by Likert scale that gave to the learners and teachers. Between group and within group investigated for both groups of participants. In general the results show the learnersʹ positive attitude toward using self-regulation and advantages of E-learning in English classrooms. The results imply the learnersʹ autonomy in learning process, and the teachers can control it by learnersʹ attitude.