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The aim of this study was to investigate the effect of a school-based educational intervention on safe crossing of streets in elementary school students. Methods: This was a quasi-experimental study with pre-test/ post test design. A total of 66 elementary school students were randomly selected from two selected schools in Yazd, Iran in 2019. The intervention consisted of theoretical and practical education in the school context. Data were collected by a self-designed checklist containing 12 questions. Data analysis was performed using spss21 software. Results: The results showed that the least safe behavior was related to pedestrian crosswalk and pedestrian bridge crossing. The mean score of street safe behavior before and after the training was 9.24(2) and 11.62(0.75), respectively, which was statistically significant (p <0.001). The mean score of safe traffic behaviors of girls after intervention was 11.7(0.48) and for boys was 11.45(0.93), which were statistically significant (p = 0.017). Conclusion: The results showed that the school-based educational intervention was effective on the safe crossing of streets in students.