Lake Puma Yumco is a typical alpine lake (altitude 5030 m) located in the pre-Himalayas of Tibet, China, and this study was the first limnological investigation ever conducted on it. Lake Puma Yumco (28°34ЈN, 90°24ЈE) has the following morphometric properties: maximum length 31 km, maximum width 14 km, mean width 9 km, shoreline 90 km, surface area 280 km 2 , and shoreline development 1.5. Transparency was approximately 10 m, even in the thawing season. The extinction coefficient of the lake water was calculated as 0.15 m Ϫ1 . Annual maximum transparency was estimated from the depth of the Chara zone to be 30 m. Dissolved oxygen was 7 mg O 2 l Ϫ1 and showed saturated values, and salinity was 360 mg l Ϫ1 . The chemical type of the lake water was Mg-Ca-HCO 3 -SO 4 , and it was slightly alkaline in character. Total nitrogenous nutrients (sum of ammonia, nitrite, nitrate, and urea nitrogen), phosphate, and silicate were extremely low at 1, 0.02, and 9 µM, respectively. Dissolved organic carbon, nitrogen, and phosphorus concentrations were 160, 11, and 0.08 µM and the molar ratio was calculated as 2100 : 140 : 1. Chlorophyll a concentration was 0.2 mg m Ϫ3 . Phytoplankton and zooplankton were dominated by Aphanocapsa sp. and Diaptomidae. Both nitrogen and phosphorus appear to be the limiting parameters for phytoplankton growth. Organic carbon and nitrogen contents in lake sediments were low and the sediments contained a large amount of CaCO 3 . The grain size of sediment was that of silt-sand in most cases. The present results indicate that the pre-Himalayan alpine freshwater Lake Puma Yumco is an ultraoligotrophic lake.

The abundance, growth, and grazing loss rates of picophytoplankton were investigated in August 2002 in Barguzin Bay, Lake Baikal. Water samples for incubation were taken once at a near-shore station and twice at an offshore station. Contributions of picophytoplankton to total phytoplankton were high (56.9-83.9%) at the offshore station and low (5.8-6.8%) at the near-shore station. The picophytoplankton community in the offshore station comprised mainly phycoerythrin (PE)-rich cyanobacteria, with eukaryotic picophytoplankton being less abundant. In contrast, as well as PE-rich cyanobacteria and eukaryotic picophytoplankton, phycocyanin (PC)-rich cyanobacteria were found in the near-shore station. At the offshore station, growth and grazing loss rates on 25 August were 0.56 and 0.43 day )1 , respectively, and on 29 August, 0.69 and 0.83 day )1 , respectively. At the near-shore station, growth and grazing loss rates were 1.61 and 0.70 day )1 , respectively. These results show that there is a difference in the abundance, composition, and ecological role in the microbial food web of picophytoplankton between the near-shore and the offshore areas in Barguzin Bay.

Nutrient limitation of the primary production of phytoplankton at some stations in southern and central Lake Baikal was studied by nutrient enrichment experiments in August 2002. Chlorophyll (Chl.) a concentrations ranged from 0.7 to 5.8 µg l −1 . Inorganic nutrient concentrations were low: soluble reactive phosphorus ranged from 0.05 to 0.20 µmol l −1 , ammonia from 0.21 to 0.41 µmol l −1 , and nitrite plus nitrate from 0.33 to 0.37 µmol l −1 . In the five enrichment experiments, phosphate spikes and phosphate plus nitrate spikes always stimulated primary production. Nitrate spikes also stimulated primary production in four of the experiments. Significant differences were detected between the controls and phosphate spikes and between the controls and phosphate plus nitrate spikes. Thus, the first limiting nutrient is thought to be phosphorus, but once phosphorus is supplied to the surface water, the limiting nutrient will quickly shift from phosphorus to nitrogen.

Dissolved silica (DSi) and its associated biological and physicochemical factors were measured in Lake Biwa, Japan and its watershed from 2002 to 2003 in order to clarify seasonal variations in the magnitude of the sink of silica and the factors that influence it within the limnetic system. Consequently, it is concluded that Lake Biwa is a noticeable body of water where a massive sink of silica is caused. Calculated silica sedimentation in Lake Biwa was 2.0 · 10 7 kg Si year À1 (7.1 · 10 8 mol Si year À1 ) which is equivalent to about 80% of the annual inflow discharge of DSi to Lake Biwa. The magnitude of the sink varies seasonally by increasing in the winter holomictic stirring period, since it is greatly affected by the species composition of phytoplankton, the load of phosphorus and the condition of stratification. It seems reasonable to suppose that the DSi in Lake Biwa is removed mainly by biological processes, i.e., the assimilation of DSi by large centric diatoms and its accumulation in their frustules. Such silica sinks occur naturally in deeper stagnant waters, providing extended water residence time and supplying a certain amount of nutrients. These findings indicate that an increase in nutrient loads and abundance of stagnant water due to the construction of large dams lead to an expansion in the magnitude of the silica sink in a limnetic system.

No abstract