Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.

In order to make use of ocean renewable energy, a combination system of a bi-directional impulse turbine and a bi-directional flow collector for tidal current energy conversion is investigated in this paper. It is the advantage that this turbine system does not need an operation of orientation change according to the reversal of regular tidal orientation when fixed on the seabed. The experimental investigations by using both a circulating water tank and a towing tank showed that the turbine power output could be increased by adopting the flow collector proposed in this study. Then the flow collector with fixed spiral vane named spiral flow collector was investigated by both a circulating water tank test and CFD analysis. The experimental result of the spiral flow collector showed that the performance improvement was found on the increase of axial velocity in the turbine which contributed to the increase of the turbine power output. The results of CFD analysis showed that 180 deg of the skew angle of the fixed spiral vane was suitable in view of the angular moment at the turbine inlet in this case.

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