Increases in adhesive and invasive commensal bacteria, such as Escherichia coli, and subsequent disruption of the epithelial barrier is implicated in the pathogenesis of inflammatory bowel disease (IBD). However, the protective systems against such barrier disruption are not fully understood. Here, we show that secretion of luminal glycoprotein 2 (GP2) from pancreatic acinar cells is induced in a TNF–dependent manner in mice with chemically induced colitis. Fecal GP2 concentration is also increased in Crohn’s diease patients. Furthermore, pancreas-specific GP2-deficient colitis mice have more severe intestinal inflammation and a larger mucosal E. coli population than do intact mice, indicating that digestive-tract GP2 binds commensal E. coli, preventing epithelial attachment and penetration. Thus, the pancreas–intestinal barrier axis and pancreatic GP2 are important as a first line of defense against adhesive and invasive commensal bacteria during intestinal inflammation.

The emergence of the occipital alpha rhythm on brain electroencephalogram (EEG) is associated with brain activity in the cerebral neocortex and deep brain structures. To further understand the mechanisms of alpha rhythm power fluctuation, we performed simultaneous EEGs and functional magnetic resonance imaging recordings in human subjects during a resting state and explored the dynamic relationship between alpha power fluctuation and blood oxygenation level-dependent (BOLD) signals of the brain. Based on the frequency characteristics of the alpha power time series (APTS) during 20-minute EEG recordings, we divided the APTS into two components: fast fluctuation (0.04–0.167 Hz) and slow fluctuation (0–0.04 Hz). Analysis of the correlation between the MRI signal and each component revealed that the slow fluctuation component of alpha power was positively correlated with BOLD signal changes in the brain stem and the medial part of the thalamus and anterior cingulate cortex, while the fast fluctuation component was correlated with the lateral part of the thalamus and the anterior cingulate cortex, but not the brain stem. In summary, these data suggest that different subcortical structures contribute to slow and fast modulations of alpha spectra on brain EEG.

Although human beings cannot perceive elastic vibrations in the frequency range above 20 kHz, nonstationary sounds containing a wealth of inaudible high-frequency components (HFC) above the human audible range activate deep-lying brain structures, including the brainstem and thalamus and evoke various physiological, psychological, and behavioral responses. In the previous reports, we have called these phenomena collectively "the hypersonic effect." It remains unclear, however, if vibratory stimuli above the audible range are transduced and perceived solely via the conventional air-conducting auditory system or if other mechanisms also contribute to mediate transduction and perception. In the present study, we have examined the emergence of the hypersonic effect when inaudible HFC and audible low-frequency components (LFC) were presented selectively to the ears, the entrance of an air-conducting auditory system, or to the body surface including the head which might contain some unknown vibratory sensing mechanisms. We used two independent measurements based on differing principles; one physiological (alpha 2 frequency of spontaneous electroencephalogram [alpha-EEG]) and the other behavioral (the comfortable listening level [CLL]). Only when the listener's entire body surface was exposed to HFC, but not when HFC was presented exclusively to the air-conducting auditory system, did both the alpha-EEG and the CLL significantly increase compared to the presentation of LFC alone, that is to say, there was an evident emergence of the hypersonic effect. The present findings suggest that the conventional air-conducting auditory system alone does not bring about the hypersonic effect. We may need to consider the possible involvement of a biological system distinct from the conventional air-conducting auditory nervous system in sensing and transducing high-frequency elastic vibration above the human audible range.

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