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The nasal output of nitric oxide (NO) is known to be high, but there have been varying reports of the exact level. We attempted to establish a quantitative measurement of nasal NO, and looked for a possible relationship with nasal resistance, at rest and during exercise.Nasal airway ventilation was performed by using an air pump at a constant flow rate, whilst the soft palate was elevated voluntarily. In a preliminary study, the flow rate for sampling was changed and concentrations of NO were measured. After determination of flow rate, rhinomanometry for nasal resistance and measurement of nasal NO by chemiluminescence were carried out before and after moderate exercise.The These data indicate that nasal NO can be measured quantitatively as V 'NO and might be involved in the control of nasal resistance. Eur Respir J., 1996, 9, 556- [6]. However, quantitative measurement has been performed in only a few studies on exhaled NO [7,8] and nasal NO [9]. In the nasal airway, NO was first detected by ALVING et al. [2] (1993). These authors found that most exhaled NO comes from the nose because of some 10 fold higher concentration of nasal NO than of exhaled NO. In general, a concentration of a gas rising from the wall of the lumen should vary with the amount of ventilation. Accordingly, the concentration of exhaled NO has been reported to correlate hyperbolically with a ventilatory volume, whereas the net output of NO (V'NO) remained constant [8]. We applied the same method in attempting to establish a quantitative measurement of the nasal airway NO, and investigated the relationship between nasal airway NO and the nasal resistance before and after exercise. Materials and methods Subjects and protocolTwelve male subjects (aged 27-35 years), with no allergies or abnormal rhinoscopic findings, volunteered for this study. The protocol of the study was reviewed and approved by the Asahikawa Medical College Institutional Review Board for Research. Subjects rested in a sitting position for at least 15 min, during the last 5 min of which rhinomanometry and the measurement of nasal airway NO were performed. Subjects then walked on a 10°graded treadmill at 5 km·h -1 for 4 min. Immediately after exercise, subjects sat down and the measurements were repeated. RhinomanometryThe anterior method of rhinomanometry [10] was applied to obtain nasal resistance of each side of the nasal airway. From the flow-pressure curve obtained by rhinomanometry (model SR-11, Rion, Japan), nasal resistance at 100 Pa was taken as the nasal resistance value (Pa·s·cm -3 ) for each subject. The total nasal resistance for both nasal airways was obtained by calculating the sum of the reciprocals of parallel resistance for each nasal airway. Measurement of nasal airway NOThe quantitative measurement of NO in the human exhaled gas has already been established [8]:

No abstract

Diffuse panbronchiolitis (DPB) is a pulmonary disease of unknown origin with inflammation in the respiratory bronchioles, bronchiectasis, and recurrent sinusitis. Patients with DPB suffer from chronic airway infections resulting from mucociliary dysfunction. Whereas a high concentration of nasal nitric oxide (NO) has been documented in healthy subjects, only two diseases are known to reduce nasal NO: primary ciliary dyskinesia syndrome and cystic fibrosis. We hypothesized that patients with DPB have abnormal levels of nasal NO. To test our hypothesis, we measured NO with the chemiluminescence technique. Air was sampled directly from the nose in 15 healthy subjects and eight patients with DPB. Nasal NO was 88% lower in DPB patients than in the age-matched control subjects (69 +/- 70 versus 556 +/- 87 nl/min; p < 0.001). Treatment with erythromycin for 2 wk did not alter the nasal NO in four control subjects. DPB is the third pulmonary disease in which nasal NO is low. The reduced nasal NO may well be involved in the pathogenesis of DPB, and NO measurements may serve as a noninvasive test in the diagnosis of DPB.