No abstract

Background: Surgical mortality data are collected routinely in high-income countries, yet virtually no low-or middle-income countries have outcome surveillance in place. The aim was prospectively to collect worldwide mortality data following emergency abdominal surgery, comparing findings across countries with a low, middle or high Human Development Index (HDI).Methods: This was a prospective, multicentre, cohort study. Self-selected hospitals performing emergency surgery submitted prespecified data for consecutive patients from at least one 2-week interval during July to December 2014. Postoperative mortality was analysed by hierarchical multivariable logistic regression.

Records of 50 dogs with signs of retrobulbar disease that had ultrasonography and diagnosis based on results of cytopathology or histopathology were reviewed retrospectively. Abnormalities were identified ultrasonographically in 43 (86%) dogs. Mass effect was identified in 42% of dogs with neoplasia and 30% of dogs with abscess. Ten of 11 (91%) orbital bone lesions were in dogs with retrobulbar neoplasia. A cavitary lesion was recognized ultrasonographically in 75% of dogs with salivary mucocele and 50% of dogs with retrobulbar abscess. Neoplasms occurred predominantly on the ventral or medial aspect of the orbit, or both, which reflects invasion by nasal or sinus neoplasms. Presence of orbital bone lesions or a mass on the medial aspect of the orbit suggests neoplasia; however, other ultrasonographic signs were nonspecific.

Summary:This randomized, controlled study compared the ability to mobilize and collect an optimal target yield of 5 × 10 6 CD34 + cells/kg using stem cell factor (SCF; 20 g/ kg/day) plus filgrastim (G-CSF; 10 g/kg/day) vs filgrastim alone (10 g/kg/day) in 102 patients diagnosed with non-Hodgkin's lymphoma (NHL) or Hodgkin's disease (HD), who were prospectively defined as being heavily pretreated. Leukapheresis began on day 5 of cytokine administration and continued daily until the target yield was reached, or until a maximum of five leukaphereses had been performed. Compared with the filgrastim-alone group (n = 54), the SCF plus filgrastim group (n = 48) showed an increase in the proportion of patients reaching the target yield within five leukaphereses (44% vs 17%, P = 0.002); reduction in the number of leukaphereses required to reach the target yield (P = 0.003); reduction in the proportion of patients failing to reach a minimum yield of 1 x 10 6 CD34 + cells/kg to proceed to transplant (16% vs 26%, P = NS); increase in the median yield of CD34 + cells per leukapheresis (0.73 × 10 6 /kg vs 0.48 × 10 6 /kg, P = 0.04); and an increase in the median total CD34 + cells collected within five leukaphereses (3.6 × 10 6 /kg vs 2.4 × 10 6 /kg, P = 0.05). All patients receiving SCF were premedicated (antihistamines and albuterol), and treatment was generally well tolerated. Five patients experienced severe mast cell-mediated reactions, none of which were life-threatening. In this study of heavily pretreated lymphoma patients, SCF plus filgrastim was more effective than filgrastim alone for mobilizing PBPC for harvesting and transplantation after high-dose chemotherapy. Bone Marrow Transplantation (2000) 26, 471-481.

The grasshopper Chorthippus parallelus is genetically differentiated over its range into at least five major geographical subdivisions. Two of these subdivisions, designated as subspecies, meet and form a hybrid zone in the Pyrenees. These subspecies differ for a wide range of morphological, behavioural and chromosomal characters, which vary clinally across the zone. A further geographical subdivision exists within Italy. Here we present the first evidence for chromosomal divergence between populations of the grasshopper north and south of the Alps. Across two Alpine cols (Col de Larche, between France and Italy; Passo de Resia, between Austria and Italy), these populations differ in both structural and functional components of the X‐chromosome. Northern Alpine individuals possess an active nucleolar organizing region (NOR) at the distal end of the X‐chromosome and an associated region of heterochromatin (C‐band). Both these features are absent from individuals from the south of the Alps. However, all individuals examined carry distally located rDNA on the X‐chromosome. Clinal transition was examined in the distal C‐band in transects through the two cols. The cline centres are roughly coincident with the tops of the cols. Both clines were of similar widths (Col de Larche, 21.88 km; Passo de Resia, 24.05 km), and therefore much wider than those for an X‐linked distal C‐band in the Pyrenean hybrid zone. This suggests that there are different selective pressures on the cytogenetic characters in the Alps. The results are discussed in the context of the historical population dynamics of the species in relation to the climatic changes associated with the Pleistocene ice ages.