Behçet's disease (BD) is a chronic multisystem vasculitis disease that can affect any organ and usually is combined with hyperactivation of neutrophils. Involvement of inflammatory cytokines such as interleukin (IL)-12 in BD has been shown before. However, IL-12 shares a p40 subunit with IL-23, which has additional inflammatory effects apart of IL-12. IL-23 increases neutrophils' transmigration and therefore could contribute in BD induction or progression. Moreover, endothelial cells express vascular endothelial cadherin adhesion molecule (VE-cadherin), which plays critical roles in angiogenesis and endothelial integrity. VE-cadherin may shed into the circulation in a soluble form (sVE-cadherin), and inflammatory cytokines can increase this process. Therefore, a correlation between IL-23 concentration and amount of sVE-cadherin was proposed. We enrolled 44 healthy persons and 53 patients with BD of different disease activities and examined their serum concentrations of IL-23 and sVE-cadherin. A significant correlation was found between the concentrations of these two factors among patients only. Comparing sVE-cadherin mean concentration in patients and controls showed a significant difference, which for IL-23 was not considered significant. Results showed higher IL-23 in sera of patients with uveitis. Moreover, there was a meaningful correlation between IL-23 content and disease activity. These results could extend the biological effects of IL-23 in BD and introduce sVE-cadherin as a potential new biomarker in the course of BD pathogenesis.

Behçet's disease (BD) is a chronic multisystem vasculitis disease that can affect any organ and usually is combined with hyperactivation of neutrophils. Involvement of inflammatory cytokines such as interleukin (IL)-12 in BD has been shown before. However, IL-12 shares a p40 subunit with IL-23, which has additional inflammatory effects apart of IL-12. IL-23 increases neutrophils' transmigration and therefore could contribute in BD induction or progression. Moreover, endothelial cells express vascular endothelial cadherin adhesion molecule (VE-cadherin), which plays critical roles in angiogenesis and endothelial integrity. VE-cadherin may shed into the circulation in a soluble form (sVE-cadherin), and inflammatory cytokines can increase this process. Therefore, a correlation between IL-23 concentration and amount of sVE-cadherin was proposed. We enrolled 44 healthy persons and 53 patients with BD of different disease activities and examined their serum concentrations of IL-23 and sVE-cadherin. A significant correlation was found between the concentrations of these two factors among patients only. Comparing sVE-cadherin mean concentration in patients and controls showed a significant difference, which for IL-23 was not considered significant. Results showed higher IL-23 in sera of patients with uveitis. Moreover, there was a meaningful correlation between IL-23 content and disease activity. These results could extend the biological effects of IL-23 in BD and introduce sVE-cadherin as a potential new biomarker in the course of BD pathogenesis.

The present study aim was to assess changes in clinical signs and plasma cytokines in calves experimentally infected with Escherchia coli and use them as a tool to diagnose colisepticemia. Ten healthy Holstein-Frisian bull calves were used for this experimental study. Experimental colisepticemia was induced in calves with intravenous injection of 1.5 × 109 CFU of O111:H8 strain of E.coli. Clinical scores were recorded before induction of septicemia, every 30 minutes for 8 hours, every hour from 8 till 12 h, every 3 hours from 12 till 24 hours after bacterial challenge. Blood samples were collected to determine plasma concentration of tumor necrosis factor-α (TNF-α) and gamma interferon gamma (IFN-γ) before and after E.coli IV injection. Blood culture was performed before and after bacterial challenge to confirm colisepticemia. The present study showed that total clinical score of the calves increased with a simultaneous significant rise in plasma concentration of TNF-α and INF-γ during septicemia period (P< 0.05). Changes in the heart and respiratory rate during septicemia and using clinical scoring are not enough to assess the magnitude of infection and disease progress; therefore, it is recommended some laboratory tests be used for better evaluation of clinical status of the septic calves