In this study, morphanatomical study and antibacterial activity of the rhizome of Zingiber officinale (Zingiberaceae) were investigated to assist as a relevant source of information and contribute towards the standards to dispose the quality and identity of this plant to avoid adulterations. The transverse section of the rhizome includes different types of tissues which are epidermis, cork, cortex and vascular bundles. The cortex is formed of parenchyma cells, which often contain starch and oils in large amounts. In vitro antibacterial activity was investigated by cork porous method. The most sensitive bacteria for all concentrations of the methanolic and only 50 mg/ml for the water extract was Staphylococcus aureus. Pseudomonas aeruginosa was found to be sensitive for both extracts used. Bacillus subtilis was sensitive for the methanolic extract only. Escherichia coli was found to be not sensitive except for 100 mg/ml concentration of the methanolic extract.