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Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.