Rabies is a viral zoonosis with 61,000 estimated deaths per year. Rabies vaccine is the best treatment pre and post exposure and virus can be produced in animal cells for veterinary use. This work had two objectives. The first one was to test three viral inocula (MOI 0.01, MOI 0.03 and 1% of working volume) in BHK-21 cells cultivated in 1.5 L spinner, comparing kinects and virus titration to determine the best inoculum. The second one was to freeze dry 12 formulations of excipients with an inactivated viral suspension, comparing visual aspects and residual moisture to select the best formulations. The best inoculum was 1% of working volume and the best excipients were trehalose, sucrose, dextran and mannitol.

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Point-of-care testing (POCT) offers several advantages over traditional laboratory testing. Offering less invasive testing with a faster turnaround time is not enough if not associated with an acceptable level of accuracy. Here, we show the analytical validation behind the multi-analyte POCT immunochromatography analyser, Hilab Flow (HiF). Analyses from 4,518 clinical samples were compared to College of American Pathologists accredited laboratories for ten quantitative and thirteen qualitative exams. Compatibility between methods was evaluated in terms of association/correlation and clinical agreement. Strong correlation/ concordance was observed between quantitative (CHOL, HDL-c, TG, HbA1c, Glycemia, 25-Hydroxy Vitamin D, TSH, Uric Acid, Creatinine, Urea) and qualitative methods (COVID-19 IgG/ IgM, Beta-hCG, Syphilis, Anti-HBsAg, Zika IgG/ IgM, Influenza A/B, HIV, HCV, HBsAg, Dengue NS1, COVID-19 Ag, Dengue IgG/ IgM, PSA). Approval criteria was obtaining a kappa agreement > 0.8 or a Pearson correlation > 0.9 depending on the exam. Overall percentage agreement was greater than 95% for all exams, indicating a good clinical agreement to gold-standard laboratory-based tests. Results indicate all exams are suitable for POCT and present a reliable performance. Data support the analyser is a useful tool to aid decision-making at the clinical setting, with potential to contribute with healthcare solutions in diagnostic medicine worldwide.