Building elements are required to provide sufficient fire resistance based on requirements set forth in the National Building Code of Canada (NBCC). Annex B of the Canadian standard for wood engineering design (CSA O86-19) provides a design methodology to calculate the structural fire-resistance of large cross-section timber elements. However, it lacks at providing design provisions for connections. The objectives of this study are to understand the fire performance of modern mass timber fasteners such as self-tapping screws, namely to evaluate their thermo-mechanical behavior and to predict their structural fire-resistance for standard fire exposure up to 2 h, as would be required for tall buildings in Canada. The results present the great fire performance of using self-tapping screws under a long time exposure on connections in mass timber construction. The smaller heated area of the exposed surface has limited thermal conduction along the fastener’s shanks and maintained their temperature profiles relatively low for two hours of exposure. Based on the heat-affected area, the study presents new design principles to determine the residual length of penetration that would provide adequate load-capacity of the fastener under fire conditions. It also allows determining safe fire-resistance values for unprotected fasteners in mass timber construction exposed up to two hours of standard fire exposure.