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A string of four new hetero binuclear Ru(III) complexes of ferrocenecarboxaldehyde-4(N)-substituted thiosemicarbazones were synthesized and characterized by various spectral (infrared, ultraviolet-visible, Electron Paramagnetic Resonance (EPR) and High Resolution Mass Spectrometry (HR-MS) techniques. The binding abilities of the ligands/complexes with nucleic acid (calf thymus DNA, CT-DNA) and bovine serum albumin (BSA) were analyzed by absorption and emission titration methods. The complexes exhibited better DNA binding affinity than their parent ligands. The interaction with CT-DNA was found to be intercalative and with BSA static quenching mechanism was observed. All the synthesized Ru(III) complexes were subjected to study their in vitro cytotoxicity against MCF-7 (human breast cancer) and HT-29 (human colon cancer) cell lines. Among the four complexes, complex 3 [RuCp (FF-etsc)PPh 3 ]Cl exhibited the highest cytotoxicity in MCF-7 cells and complex 4 [RuCp (FF-ptsc)PPh 3 ]Cl was the most active on HT-29 cells. K E Y W O R D S acridine orange-ethidium bromide (AO-EB)/4, 6-diamidino-2-phenylindole (DAPI) staining, morphological studies, MTT assay, Ru(III) complexes

Series of organoruthenium complexes and their nano biocomposites were prepared and analyzed by various spectral analyzed techniques like infrared (IR), UV-Vis, 1 H-nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) spectroscopy, and high-resolution mass spectrometry (HR-MS). The redox properties of organoruthenium complexes were studied by cyclic voltammogram. UV-Vis and fluorescence spectroscopy employed to study the binding affinity of Ru (III) complexes with CT-DNA and serum albumin (bovine serum albumin [BSA]). From the K sv and K b values, the interaction between BSA and organoruthenium complexes confirmed the mechanism of binding as static quenching. Scanning electron microscopic (SEM) studies validated the formation of new hybrid organometallic proteomics (P1-P4), while binding of the complexes with protein (BSA). A comparative cytotoxic profile of organoruthenium complexes and their nano biocomposites were evaluated. The results revealed the higher anticancer activity of nano biocomposites than their parent organoruthenium derivatives.