Large in-stream wood (LW) is a critical component of riparian systems that increases heterogeneity of flow regimes and provides high quality habitat for salmonids and other fishes. We present four sampling-based methods to estimate two-dimensional LW for a 61-hectare river restoration project on the South Fork McKenzie River near Rainbow, OR (USA). We manually delineated LW area, from unoccupied aircraft systems (UAS) multispectral imagery for 40 randomly selected 51.46 m 2 hexagonal plots. Seven auxiliary variables were extracted from the imagery and imagery derivatives to be incorporated in four estimators by summarizing spectral statistics for each plot including Random forest (RF) classification of segmented imagery (Cohen's kappa = 0.75, balanced accuracy = 0.86). The four estimators were: difference estimator, simple linear regression estimator with one auxiliary variable, general regression estimator with seven auxiliary variables, and simple random sample without replacement. We assessed variance of the estimators and found that the simple random sample without replacement produced the largest estimate for LW area and widest confidence interval (17,283 m 2 , 95% CI 10,613 -23,952 m 2 ) while the generalized regression approach resulted in the smallest estimate and narrowest confidence interval (16,593 m 2 , 95% CI 13,054 -20,133 m 2 ). These methods facilitate efficient estimates of critical habitat components, that are especially suited to efforts that seek to quantify large amounts of these components through time. When combined with traditional sampling methods, classified imagery acquired via UAS promises to enhance the temporal resolution of the data products associated with restoration efforts while minimizing the necessity for potentially hazardous field work.