Wild Mediterranean fruit fly specimens collected from various regions worldwide were screened for the glycine to alanine (Gly->Ala) point mutation (G328A) in the acetylcholinesterase enzyme, presumably causing resistance to organophosphates. We found that the single nucleotide polymorphism (SNP) responsible for this amino acid change is located at the beginning of exon 6 of the Ccace2 gene. The identification of the exact location of the SNP permitted PCR primer design around this site and direct sequencing of the corresponding genomic region. We detected the resistance allele in natural Mediterranean fruit fly populations from Brazil and Spain, but not from other sites in four continents. The known treatment history of sites suggests that the resistance buildup is linked to organophosphate application in the field. The PCR-based detection provides a screening method useful for monitoring Mediterranean fruit fly insecticide resistance in local populations and improving pest management strategies accordingly.

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Despite the nature of American influence in postwar Iran, and despite the fact that Iranian studies has grown into a flourishing field in the United States, scholars have not explored the field's origins during the Cold War era. This article begins with the life of T. Cuyler Young to trace the critical genealogy within the field as it developed, in cooperation between American and Iranian scholars, during the reign of Mohammad Reza Shah Pahlavi. It proceeds to analyze two cohorts of American scholars whose political inclinations ranged from liberal reformism to revolutionary Marxism. As revolutionary momentum swelled in Iran in the late 1970s, critical scholars broke through superpower dogmas and envisioned a post-shah Iran. However, Cold War teleologies prevented them from fully grasping Iranian realities, particularly Khomeini's vision for Iran. This article argues that the modern field of Iranian studies in the United States was shaped by multiple generations of critical voices, all of which were informed by historically situated encounters with Iran and expressed through a range of methodological and theoretical perspectives.