Changes in extracellular matrix (ECM) are one of many components that contribute to impaired wound healing in aging. This study examined the effect of age on the glycosaminoglycan hyaluronan (HA) in normal and wounded dermis from young (4–6 month-old) and aged (22–24 month-old) mice. HA content and size was similar in the normal dermis of young and aged mice. Dermal explants labeled with [3H]-glucosamine showed decreased generation of smaller forms of HA in aged explants relative to young explants. Aged mice exhibited delayed wound repair compared with young mice with the greatest differential at 5 days. Expression of hyaluronan synthase (HAS) 2,3 and hyaluronidase (HYAL) 1-3 mRNA in wounds of young and aged mice was similar. There was a trend toward decreased HYAL protein expression in aged wound dermis, which was accompanied by changes in detectable HYAL activity. Total HA content was similar in young and aged wound dermis. There was significantly less HA in the lower MW range (~250 kDa and smaller) in 5-day wound dermis, but not 9-day wound dermis, from aged mice relative to young mice. We propose that decreased cleavage of HA is an additional component of impaired dermal wound healing in aging.

Background: Sedation is an essential component of the transesophageal echocardiography (TEE) procedure for patient comfort. Utilization and the clinical implications of cardiologist-supervised (CARD-Sed) versus anesthesiologist-supervised sedation (ANES-Sed) are unknown. Methods: We reviewed non-operative TEE records from a single academic center over a five-year time period and identified CARD-Sed and ANES-Sed cases. We evaluated the impact of patient comorbidities, cardiac abnormalities on transthoracic echocardiogram (TTE), and the indication for TEE on sedation practice. We analyzed the use of CARD-Sed versus ANES-Sed in light of institutional guidelines, the consistency in the documentation of pre-procedural risk stratification, and the incidence of cardiopulmonary events including hypotension, hypoxia and hypercarbia. Results: A total of 914 patients underwent TEE, with 475 (52%) receiving CARD-Sed and 439 (48%) ANES-Sed. The presence of obstructive sleep apnea (p=0.008), a BMI greater than 45kg/m (p<0.001), an EF of less than 30% (p<0.001) and pulmonary artery systolic pressure of more than 40 mm Hg (p=0.015) were all associated with the use of ANES-Sed. Of the 178 (19.5%) patients with at least one caution to non-anesthesiologist-supervised sedation by the institutional screening guideline, 65 (36.5%) underwent CARD-Sed. In the ANES-Sed group, where intraprocedural vital signs and medications were documented in all cases, hypotension (n = 91, 20.7%), vasoactive medication use (n=121, 27.6%), hypoxia (n= 35, 8.0%), and hypercarbia (n= 50, 11.4%) were noted. Conclusions: This single-center study revealed that 56% of the non-operative TEE utilized ANES-Sed over five years. Sedation-related hemodynamic changes and respiratory events were not infrequently encountered during ANES-Sed.