The conformational heterogeneity of backbone N-substituted peptides limits their ability to adopt stable secondary structures. Herein, we describe a practical synthesis of backbone aminated peptides that readily adopt β-sheet folds. Data derived from model N-amino peptides suggest that extended conformations are stabilized through cooperative steric, electrostatic, and hydrogen-bonding interactions.
The
design and solid-phase synthesis of tetrahydropyridazine-3,6-dione
(Tpd) peptidomimetics derived from backbone-aminated peptides is reported.
The described protocol features the synthesis of chiral α-hydrazino
acids suitable for chemoselective incorporation into growing peptide
chains. Acid-catalyzed cyclization to form the Tpd ring during cleavage
affords the target peptidomimetics in good yield and purity. The scope
of Tpd incorporation is demonstrated through the synthesis of constrained
peptides featuring nucleophilic/electrophilic side chains and sterically
encumbered α-substituted hydrazino acid residues.
Short peptides featuring a tetrahydropyridazinedione (tpd) backbone tether exhibit reduced conformational flexibility external to the heterocyclic constraint. Analysis by NMR, molecular modeling and X-ray crystallography suggests both covalent and non-covalent stabilization of extended peptide conformations. An efficient solid-phase protocol was developed for the synthesis of a new class of β-strand mimics based on oligomeric tpd subunits.
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